SPR with Cynomolgus M-CSF R/CSF1R/CD115 Protein 4166

ELISA with Cynomolgus M-CSF R/CSF1R/CD115 Protein 4166

Cynomolgus M-CSF R/CSF1R/CD115 Protein 4166

$240.00 – $800.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ile99-Pro596

SKU: 4166parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	G7P8P1
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
molecular weight	The protein has a predicted MW of 56.3 kDa. Due to glycosylation, the protein migrates to 75-105 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus M-CSF R/CSF1R/CD115 Protein 4166

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CSF1R also known as M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region.CSF1R is tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes.
Protein names receptor protein-tyrosine kinase (EC 2.7.10.1)
Protein family Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamil
Mass 9541Da
Catalytic activity BINDING 640; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-3"; BINDING 667..675; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR500947-51"; BINDING 668..675; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-2"; BINDING 695; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-2, ECO:0000256\|PROSITE-ProRule:PRU10141"; BINDING 743..749; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-2"; BINDING 861; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-2"; BINDING 862; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-3"; BINDING 875; /ligand="Mg(2+)"; /ligand_id="ChEBI:CHEBI:18420"; /evidence="ECO:0000256\|PIRSR:PIRSR000615-3"
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane ; Single-pass type I membrane protein .
Target Relevance information above includes information from UniProt accession: G7P8P1
The UniProt Consortium

Data

SPR with Cynomolgus M-CSF R/CSF1R/CD115 Protein

Cynomolgus M-CSF R, His Tag immobilized on CM5 Chip can bind Cynomolgus M-CSF, His Tag with an affinity constant of 0.889 µM as determined in SPR assay (Biacore T200).

ELISA with Cynomolgus M-CSF R/CSF1R/CD115 Protein

Immobilized Cynomolgus M-CSF R, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-M-CSF R Antibody, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA (QC Test).

HPLC of Cynomolgus M-CSF R/CSF1R/CD115 Protein

The purity of Cynomolgus M-CSF R is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus M-CSF R/CSF1R/CD115 Protein

Cynomolgus M-CSF R on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.