SPR with Cynomolgus IL-3 R alpha/CD123 Protein 4175

Cynomolgus IL-3 R alpha/CD123 Protein 4175

$345.00 – $1,150.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Arg18-Arg302

SKU: 4175parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	G8F3K3
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.
molecular weight	The protein has a predicted MW of 33.9 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus IL-3 R alpha/CD123 Protein 4175

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and Type 5 subfamily.The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias.
Protein names Interleukin-3 receptor subunit alpha
Protein family Type I cytokine receptor family, Type 5 subfamily
Mass 43354Da
Subellular location Membrane ; Single-pass type I membrane protein .
Target Relevance information above includes information from UniProt accession: G8F3K3
The UniProt Consortium

Data

SPR with Cynomolgus IL-3 R alpha/CD123 Protein

Human IL-3, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus IL-3 R alpha, His Tag with an affinity constant of 0.46 µM as determined in SPR assay (Biacore T200) (QC Test).

Cynomolgus IL-3 R alpha, His Tag immobilized on CM5 Chip can bind Cynomolgus IL-3, His Tag with an affinity constant of 1.30 nM as determined in SPR assay (Biacore T200).

HPLC of Cynomolgus IL-3 R alpha/CD123 Protein

The purity of Cynomolgus IL-3 R alpha is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus IL-3 R alpha/CD123 Protein

Cynomolgus IL-3 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.