SPR with Cynomolgus Fc gamma RIIA/CD32a Protein 4180

Cynomolgus Fc gamma RIIA/CD32a Protein 4180

$240.00 – $800.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Gln28-Pro208

SKU: 4180parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q8SPW4
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
molecular weight	The protein has a predicted MW of 21.3 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus Fc gamma RIIA/CD32a Protein 4180

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
Protein names Fc gamma receptor IIa
Mass 34219Da
Target Relevance information above includes information from UniProt accession: Q8SPW4
The UniProt Consortium

Data

SPR with Cynomolgus Fc gamma RIIA/CD32a Protein

Cynomolgus Fc gamma RIIA, His Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 4.39 µM as determined in SPR assay (Biacore T200).

HPLC of Cynomolgus Fc gamma RIIA/CD32a Protein

The purity of Cynomolgus Fc gamma RIIA is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus Fc gamma RIIA/CD32a Protein

Cynomolgus Fc gamma RIIA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.