ELISA with Cynomolgus EPHB2 Protein 2790

Cynomolgus EPHB2 Protein 2790

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Val19-Pro542

SKU: 2790parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A7N9CQH5
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia.
molecular weight	The protein has a predicted MW of 59.09 kDa. Due to glycosylation, the protein migrates to 65-75 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus EPHB2 Protein 2790

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
EphB2, a receptor tyrosine kinase for ephrin ligands, is overexpressed in various cancers and plays an important role in tumor progression. EPHB2 promotes endothelial-mesenchymal transition (EMT) and elicits associated pathologic characteristics of glioblastoma multiforme (GBM) such as invasion and migration. EPHB2 is epigenetically overexpressed in hypoxia, a condition highly prevalent in malignancy. Furthermore, HIF-2α is required for EPHB2 stabilization by hypoxia.
Protein names receptor protein-tyrosine kinase (EC 2.7.10.1)
Gene names EPHB2,EPHB2
Mass 9541Da
Catalytic activity BINDING 628..636; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR000666-2"; BINDING 654; /ligand="ATP"; /ligand_id="ChEBI:CHEBI:30616"; /evidence="ECO:0000256\|PIRSR:PIRSR000666-2, ECO:0000256\|PROSITE-ProRule:PRU10141"
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane ; Single-pass type I membrane protein .
Target Relevance information above includes information from UniProt accession: A0A7N9CQH5
The UniProt Consortium

Immobilized Cynomolgus EPHB2, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-EPHB2 Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.

The purity of Cynomolgus EPHB2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus EPHB2 Protein

Cynomolgus EPHB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.