ELISA with Cynomolgus E-selectin/CD62E Protein 3111

Cynomolgus E-selectin/CD62E Protein 3111

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Trp22-Pro554

SKU: 3111parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A2K5U7H9
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 – 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
molecular weight	The protein has a predicted MW of 59.47 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus E-selectin/CD62E Protein 3111

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
E-Selectin (Endothelial Leukocyte Adhesion Molecule-1, ELAM-1, CD62E), a member of the Selectin family, is a 107 - 115 kDa cell surface glycoprotein. It is transiently expressed on vascular endothelial cells in response to IL-1 beta and TNF-alpha.E-selectin mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Protein names E-selectin (CD62 antigen-like family member E) (Endothelial leukocyte adhesion molecule 1) (Leukocyte-endothelial cell adhesion molecule 2)
Gene names SELE,SELE
Protein family Selectin/LECAM family
Mass 9541Da
Subellular location Cell membrane ; Single-pass type I membrane protein. Membrane ; Single-pass type I membrane protein .
Structure Interacts with SELPLG/PSGL1 and PODXL2 through the sialyl Lewis X epitope. SELPLG sulfation appears not to be required for this interaction.
Target Relevance information above includes information from UniProt accession: A0A2K5U7H9
The UniProt Consortium

Data

ELISA with Cynomolgus E-selectin/CD62E Protein

Immobilized Cynomolgus E-selectin, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Anti-E-Selectin Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA.

HPLC of Cynomolgus E-selectin/CD62E Protein

The purity of Cynomolgus E-selectin is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus E-selectin/CD62E Protein

Cynomolgus E-selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.