SPR with Cynomolgus DNAM-1/CD226 Protein 4266

ELISA with Cynomolgus DNAM-1/CD226 Protein 4266

Cynomolgus DNAM-1/CD226 Protein 4266

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu19-Phe252

SKU: 4266parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A2K5W1Z6
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
molecular weight	The protein has a predicted MW of 27.7 kDa. Due to glycosylation, the protein migrates to 48-68 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus DNAM-1/CD226 Protein 4266

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
DNAX accessory molecule-1 (DNAM-1), also known as CD226, is a 65 kDa type I transmembrane glycoprotein in the immunoglobulin superfamily.DNAM-1 mediates cellular adhesion to other cells bearing its ligands, CD112 and CD155, and cross-linking DNAM-1 with antibodies causes cellular activation. Furthermore, DNAM-1 can interact with PVR and PVRL2.
Protein names CD226 molecule
Gene names CD226,CD226
Mass 38545Da
Target Relevance information above includes information from UniProt accession: A0A2K5W1Z6
The UniProt Consortium

Data

SPR with Cynomolgus DNAM-1/CD226 Protein

Human CD155, hFc Tag captured on CM5 Chip via Protein A can bind Cynomolgus DNAM-1, His Tag with an affinity constant of 0.82 µM as determined in SPR assay (Biacore T200).

ELISA with Cynomolgus DNAM-1/CD226 Protein

Immobilized Cynomolgus DNAM-1, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Human CD155, mFc Tag with the EC50 of 1.63µg/ml determined by ELISA (QC Test).

The purity of Cynomolgus DNAM-1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus DNAM-1/CD226 Protein

Cynomolgus DNAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.