Cynomolgus CD98 Protein 2212

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Arg175-Ala599

SKU: 2212parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	XP_045227941
express system	HEK293
product tag	N-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The type II transmembrane protein CD98, best known as the heavy chain of the heterodimeric amino acid transporters (HAT), is required for the surface expression and basolateral localization of this transporter complex in polarized epithelial cells. CD98 also interacts with beta1 integrins resulting in an increase in their affinity for ligand. In this study we explored the role of the transmembrane and cytoplasmic domains of CD98 on integrin-dependent cell adhesion and migration in polarized renal epithelial cells.
molecular weight	The protein has a predicted MW of 48.02 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus CD98 Protein 2212

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The type II transmembrane protein CD98, best known as the heavy chain of the heterodimeric amino acid transporters (HAT), is required for the surface expression and basolateral localization of this transporter complex in polarized epithelial cells. CD98 also interacts with beta1 integrins resulting in an increase in their affinity for ligand. In this study we explored the role of the transmembrane and cytoplasmic domains of CD98 on integrin-dependent cell adhesion and migration in polarized renal epithelial cells.
Protein names Amino acid transporter heavy chain SLC3A2 (4F2 cell-surface antigen heavy chain) (4F2hc) (4F2 heavy chain antigen) (Lymphocyte activation antigen 4F2 large subunit) (Solute carrier family 3 member 2) (CD antigen CD98)
Protein family SLC3A transporter family
Mass 67994Da
Function Acts as a chaperone that facilitates biogenesis and trafficking of functional transporters heterodimers to the plasma membrane. Forms heterodimer with SLC7 family transporters (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10 and SLC7A11), a group of amino-acid antiporters (PubMed:10574970, PubMed:10903140, PubMed:11557028, PubMed:30867591, PubMed:33298890, PubMed:33758168, PubMed:34880232, PubMed:9751058, PubMed:9829974, PubMed:9878049). Heterodimers function as amino acids exchangers, the specificity of the substrate depending on the SLC7A subunit. Heterodimers SLC3A2/SLC7A6 or SLC3A2/SLC7A7 mediate the uptake of dibasic amino acids (PubMed:10903140, PubMed:9829974). Heterodimer SLC3A2/SLC7A11 functions as an antiporter by mediating the exchange of extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane (PubMed:34880232). SLC3A2/SLC7A10 translocates small neutral L- and D-amino acids across the plasma membrane (By similarity). SLC3A2/SLC75 or SLC3A2/SLC7A8 translocates neutral amino acids with broad specificity, thyroid hormones and L-DOPA (PubMed:10574970, PubMed:11389679, PubMed:11557028, PubMed:11564694, PubMed:11742812, PubMed:12117417, PubMed:12225859, PubMed:12716892, PubMed:15980244, PubMed:30867591, PubMed:33298890, PubMed:33758168). SLC3A2 is essential for plasma membrane localization, stability, and the transport activity of SLC7A5 and SLC7A8 (PubMed:10391915, PubMed:10574970, PubMed:11311135, PubMed:15769744, PubMed:33066406). When associated with LAPTM4B, the heterodimer SLC7A5 is recruited to lysosomes to promote leucine uptake into these organelles, and thereby mediates mTORC1 activation (PubMed:25998567). Modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration and tumor progression (PubMed:11121428, PubMed:15625115). {ECO:0000250\|UniProtKB:P63115, ECO:0000269\|PubMed:10391915, ECO:0000269\|PubMed:10574970, ECO:0000269\|PubMed:10903140, ECO:0000269\|PubMed:11121428, ECO:0000269\|PubMed:11311135, ECO:0000269\|PubMed:11389679, ECO:0000269\|PubMed:11557028, ECO:0000269\|PubMed:11564694, ECO:0000269\|PubMed:11742812, ECO:0000269\|PubMed:12117417, ECO:0000269\|PubMed:12225859, ECO:0000269\|PubMed:12716892, ECO:0000269\|PubMed:15625115, ECO:0000269\|PubMed:15769744, ECO:0000269\|PubMed:15980244, ECO:0000269\|PubMed:25998567, ECO:0000269\|PubMed:30867591, ECO:0000269\|PubMed:33066406, ECO:0000269\|PubMed:33298890, ECO:0000269\|PubMed:33758168, ECO:0000269\|PubMed:34880232, ECO:0000269\|PubMed:9751058, ECO:0000269\|PubMed:9829974, ECO:0000269\|PubMed:9878049}.; (Microbial infection) In case of hepatitis C virus/HCV infection, the complex formed by SLC3A2 and SLC7A5/LAT1 plays a role in HCV propagation by facilitating viral entry into host cell and increasing L-leucine uptake-mediated mTORC1 signaling activation, thereby contributing to HCV-mediated pathogenesis. {ECO:0000269\|PubMed:30341327}.; (Microbial infection) Acts as a receptor for malaria parasite Plasmodium vivax (Thai isolate) in immature red blood cells. {ECO:0000269\|PubMed:34294905}.
Subellular location Apical cell membrane {ECO:0000269\|PubMed:11742812}. Cell membrane {ECO:0000269\|PubMed:11311135, ECO:0000269\|PubMed:11389679, ECO:0000269\|PubMed:11557028, ECO:0000269\|PubMed:11564694, ECO:0000269\|PubMed:12225859, ECO:0000269\|PubMed:15769744, ECO:0000269\|PubMed:16496379, ECO:0000269\|PubMed:25998567, ECO:0000269\|PubMed:3476959, ECO:0000269\|PubMed:3480538, ECO:0000269\|PubMed:36028562, ECO:0000269\|PubMed:9829974}; Single-pass type II membrane protein {ECO:0000269\|PubMed:30867591}. Cell junction {ECO:0000250\|UniProtKB:P10852}. Lysosome membrane {ECO:0000269\|PubMed:25998567}. Melanosome {ECO:0000269\|PubMed:17081065}. Basolateral cell membrane {ECO:0000250\|UniProtKB:P10852}. Note=Localized at the plasma membrane when associated with SLC7A5/LAT1 or SLC7A8/LAT2 (PubMed:11311135, PubMed:9751058). Localized to the apical membrane of placental syncytiotrophoblastic cells (PubMed:11742812). Recruited to lysosomes by LAPTM4B (PubMed:25998567). Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Located selectively at cell-cell adhesion sites (By similarity). Colocalized with SLC7A8/LAT2 at the basolateral membrane of kidney proximal tubules and small intestine epithelia. Expressed in both luminal and abluminal membranes of brain capillary endothelial cells (By similarity). {ECO:0000250\|UniProtKB:P10852, ECO:0000269\|PubMed:11311135, ECO:0000269\|PubMed:11742812, ECO:0000269\|PubMed:17081065, ECO:0000269\|PubMed:25998567, ECO:0000269\|PubMed:9751058}.
Tissues Expressed ubiquitously in all tissues tested with highest levels detected in kidney, placenta and testis and weakest level in thymus. During gestation, expression in the placenta was significantly stronger at full-term than at the mid-trimester stage. Expressed in HUVECS and at low levels in resting peripheral blood T-lymphocytes and quiescent fibroblasts. Also expressed in fetal liver and in the astrocytic process of primary astrocytic gliomas. Expressed in retinal endothelial cells and in the intestinal epithelial cell line C2BBe1. {ECO:0000269\|PubMed:11389679, ECO:0000269\|PubMed:11557028, ECO:0000269\|PubMed:11742812, ECO:0000269\|PubMed:12716892, ECO:0000269\|PubMed:14603368, ECO:0000269\|PubMed:15980244, ECO:0000269\|PubMed:16496379, ECO:0000269\|PubMed:3265470, ECO:0000269\|PubMed:3480538}.
Structure Disulfide-linked heterodimer with a non-glycosylated catalytic light subunit (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10 or SLC7A11) (PubMed:10574970, PubMed:10903140, PubMed:11311135, PubMed:11557028, PubMed:12117417, PubMed:12225859, PubMed:15769744, PubMed:30867591, PubMed:33298890, PubMed:33758168, PubMed:34880232, PubMed:35352032, PubMed:9751058, PubMed:9829974). Interacts with TLCD3A/CT120 (PubMed:12270127). Interacts with ICAM1 (PubMed:12716892). Constitutively and specifically associates with beta-1 integrins (alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1 and alpha-6/beta-1), but minimally with alpha-4/beta-1 (PubMed:11696247). Interacts with LAPTM4B; recruits SLC3A2 and SLC7A5/LAT1 to lysosomes to promote leucine uptake into these organelles and is required for mTORC1 activation (PubMed:25998567). {ECO:0000269\|PubMed:10574970, ECO:0000269\|PubMed:10903140, ECO:0000269\|PubMed:11311135, ECO:0000269\|PubMed:11389679, ECO:0000269\|PubMed:11557028, ECO:0000269\|PubMed:11564694, ECO:0000269\|PubMed:11696247, ECO:0000269\|PubMed:12117417, ECO:0000269\|PubMed:12225859, ECO:0000269\|PubMed:12270127, ECO:0000269\|PubMed:12716892, ECO:0000269\|PubMed:14603368, ECO:0000269\|PubMed:15769744, ECO:0000269\|PubMed:17724034, ECO:0000269\|PubMed:25998567, ECO:0000269\|PubMed:30867591, ECO:0000269\|PubMed:33298890, ECO:0000269\|PubMed:33758168, ECO:0000269\|PubMed:34880232, ECO:0000269\|PubMed:35352032, ECO:0000269\|PubMed:9751058, ECO:0000269\|PubMed:9829974, ECO:0000269\|PubMed:9878049}.; (Microbial infection) Interacts with hepatitis C virus/HCV envelope glycoprotein E2; the interaction may facilitate viral entry into host cell. {ECO:0000269\|PubMed:30341327}.
Post-translational modification N-glycosylated; N-glycosylation is crucial for trafficking and stability of SLC3A2 to the plasma membrane. {ECO:0000269\|PubMed:36028562}.; Phosphorylation on Ser-406; Ser-408 or Ser-410 and on Ser-527 or Ser-531 by ecto-protein kinases favors heterotypic cell-cell interactions. {ECO:0000269\|PubMed:19065266}.
Target Relevance information above includes information from UniProt accession: P08195
The UniProt Consortium

Immobilized Cynomolgus CD98, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Anti-CD98 Antibody, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA.

The purity of Cynomolgus CD98 is greater than 95% as determined by SEC-HPLC.

Cynomolgus CD98 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.