ELISA with Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein 4544

Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein 4544

$330.00 – $1,100.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gln22-Asp117(CD3E) & Gln23-Thr113(CD3G)

SKU: 4544parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	Q95LI5.2(CD3E)&Q95LI7(CD3G)
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G, are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
molecular weight	The protein has a predicted MW of 36.9 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 44-46 kDa&46-48 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein 4544

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

ELISA with Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein

Immobilized Cynomolgus CD3E&CD3G, hFc Tag at 0.5µg/ml (100µl/Well). Dose response curve for Biotinylated Anti-CD3 Antibody, hFc Tag with EC50 of 20.1ng/ml determined by ELISA.

HPLC of Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein

The purity of Cynomolgus CD3E&CD3G was greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus CD3E&CD3G/CD3 epsilon&CD3 gamma Protein

Cynomolgus CD3E&CD3G on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.