ELISA with Cynomolgus CD27/TNFRSF7 Protein 3089

Cynomolgus CD27/TNFRSF7 Protein 3089

$270.00 – $900.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Thr21-Ile192

SKU: 3089parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	XP_005569963
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD27, also known as TNFRSF7, is an approximately 55 kDa transmembrane protein in the TNF receptor superfamily. It functions as a co‑stimulatory molecule that supports lymphocyte activation and survival. It binds to ligand CD70, and plays a key role in regulating B-cell activation and immunoglobulin synthesis.
molecular weight	The protein has a predicted MW of 20.41 kDa. Due to glycosylation, the protein migrates to 42-52 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus CD27/TNFRSF7 Protein 3089

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

ELISA with Cynomolgus CD27/TNFRSF7 Protein

Immobilized Cynomolgus CD27, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human CD27 Ligand, hFc Tag with the EC50 of 17.6ng/ml determined by ELISA.

The purity of Cynomolgus CD27 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus CD27/TNFRSF7 Protein

Cynomolgus CD27 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

1. Q: What is the molecular weight of the Cynomolgus CD27 (TNFRSF7) protein product (3089)?
A: The molecular weight of the Cynomolgus CD27 (TNFRSF7) protein is approximately 19.5 kDa.

2. Q: How should I store the Cynomolgus CD27 (TNFRSF7) protein for optimal preservation?
A: The protein should be stored at -20°C upon arrival. For long-term storage, it is recommended to store it at -80°C. Avoid repeated freeze-thaw cycles to maintain protein integrity.

3. Q: Is this Cynomolgus CD27 (TNFRSF7) protein suitable for use in human studies or experiments?
A: While this protein is derived from cynomolgus monkeys, it shares high sequence similarity with the human CD27 protein, making it compatible with human research applications, including binding studies, functional assays, and more.

4. Q: Can this Cynomolgus CD27 (TNFRSF7) protein be used for flow cytometry experiments?
A: Yes, this protein is suitable for flow cytometry experiments, allowing for the detection and analysis of CD27 expression on target cells.

5. Q: What are some potential applications for the Cynomolgus CD27 (TNFRSF7) protein in research?
A: This protein can be used in various research applications, including cell signaling studies, ligand-receptor interaction assays, immunoprecipitation experiments, and as a positive control in Western blotting.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.