SPR with Cynomolgus CD200 R1/CRTR2 Protein 3203

ELISA with Cynomolgus CD200 R1/CRTR2 Protein 3203

Cynomolgus CD200 R1/CRTR2 Protein 3203

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu25-Leu267

SKU: 3203parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	XP_005548207
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.
molecular weight	The protein has a predicted MW of 28.09 kDa. Due to glycosylation, the protein migrates to 67-77 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus CD200 R1/CRTR2 Protein 3203

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
CD200Fc, a chimeric molecule including the extracellular domain of CD200 and a murine IgG2a Fc region, regulates immune responses following engagement of a cell surface receptor, CD200R, expressed on cells of the myeloid and T cell lineage. A recent report focused attention on a family of CD200Rs, but concluded that only one member used CD200 as its ligand.
Protein names Cell surface glycoprotein CD200 receptor 1 (CD200 cell surface glycoprotein receptor) (Cell surface glycoprotein OX2 receptor 1)
Protein family CD200R family
Mass 39041Da
Function Inhibitory receptor for the CD200/OX2 cell surface glycoprotein. Limits inflammation by inhibiting the expression of pro-inflammatory molecules including TNF-alpha, interferons, and inducible nitric oxide synthase (iNOS) in response to selected stimuli. Also binds to HHV-8 K14 viral CD200 homolog with identical affinity and kinetics as the host CD200. {ECO:0000269\|PubMed:12960329}.
Subellular location [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 4]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Isoform 3]: Secreted.
Tissues Expressed in granulocytes, monocytes, most T-cells, neutrophils, basophils and a subset of NK, NKT and B-cells (at protein level). Expressed in bone marrow, lymph nodes, spleen, lung, liver, spinal cord, kidney. Expressed in monocyte-derived dendritic and mast cells. {ECO:0000269\|PubMed:12960329}.
Structure CD200 and CD200R1 interact via their respective N-terminal Ig-like domains (By similarity). Interacts with Human herpesvirus 8 vOX2 protein. {ECO:0000250}.; (Microbial infection) Interacts with human herpesvirus 8/HHV-8 protein vOX2/K14. {ECO:0000269\|Ref.2}.
Target Relevance information above includes information from UniProt accession: Q8TD46
The UniProt Consortium

Data

SPR with Cynomolgus CD200 R1/CRTR2 Protein

Cynomolgus CD200 R1, His Tag immobilized on CM5 Chip can bind Cynomolgus CD200, His Tag with an affinity constant of 35.84 nM as determined in SPR assay (Biacore T200).

ELISA with Cynomolgus CD200 R1/CRTR2 Protein

Immobilized Cynomolgus CD200 R1, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Human CD200, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA (QC Test).

HPLC of Cynomolgus CD200 R1/CRTR2 Protein

The purity of Cynomolgus CD200 R1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus CD200 R1/CRTR2 Protein

Cynomolgus CD200 R1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Database connection successful.

Frequently Asked Questions

1. What is the purity of the Cynomolgus CD200-R1 (CRTR2) protein offered on the website?
- The Cynomolgus CD200-R1 (CRTR2) protein available on our website has a purity of > 95%.

2. How should the Cynomolgus CD200-R1 (CRTR2) protein be stored for optimal preservation?
- To maintain its stability, we recommend storing the Cynomolgus CD200-R1 (CRTR2) protein at -20°C upon arrival. Avoid repeated freeze-thaw cycles to prevent degradation.

3. Is this Cynomolgus CD200-R1 (CRTR2) protein compatible with various experimental techniques?
- Yes, this protein is suitable for various applications such as ELISA, Western blotting, immunoprecipitation, and flow cytometry.

4. What are the potential research applications of the Cynomolgus CD200-R1 (CRTR2) protein?
- The Cynomolgus CD200-R1 (CRTR2) protein can be used in studies related to immunology, inflammation, and neurobiology. It can also be utilized in cell signaling and receptor-ligand interaction research.

5. Can this protein be used in functional assays or cellular studies?
- Yes, researchers can utilize this Cynomolgus CD200-R1 (CRTR2) protein to investigate its function in cellular assays, such as ligand-receptor binding studies or signal transduction pathways involved in immune responses.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

We havent added any publications to our database yet.