SPR with Cynomolgus B7-1/CD80 Protein 5175

ELISA with Cynomolgus B7-1/CD80 Protein 5175

Cynomolgus B7-1/CD80 Protein 5175

$240.00 – $800.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Val35-Asn242

SKU: 5175parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	G7NXN7
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
molecular weight	The protein has a predicted MW of 25 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus B7-1/CD80 Protein 5175

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Cluster of differentiation 80 (also CD80 and B7-1) is a protein found on dendritic cells, activated B cells and monocytes that provides a costimulatory signal necessary for T cell activation and survival. It is the ligand for two different proteins on the T cell surface: CD28 and CTLA-4.
Protein names CD80 molecule
Gene names CD80,CD80
Mass 9541Da
Subellular location Membrane ; Single-pass type I membrane protein .
Target Relevance information above includes information from UniProt accession: G7NXN7
The UniProt Consortium

Data

Cynomolgus B7-1, His Tag immobilized on CM5 Chip can bind Cynomolgus CTLA-4, His Tag with an affinity constant of 9.90 nM as determined in SPR assay (Biacore T200).

Immobilized Human CTLA-4, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Cynomolgus B7-1, His Tag with the EC50 of 0.23µg/ml determined by ELISA (QC Test).

The purity of Cynomolgus B7-1 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus B7-1/CD80 Protein

Cynomolgus B7-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.