ELISA with Cynomolgus 4-1BB Ligand/TNFSF9 Protein 4258

Cynomolgus 4-1BB Ligand/TNFSF9 Protein 4258

$315.00 – $1,050.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Arg68-Glu251

available size

Clear

SKU: 4258parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	XP_015296398
express system	HEK293
product tag	C-hFc
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.
molecular weight	The protein has a predicted MW of 46 kDa. Due to glycosylation, the protein migrates to 50-52 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Cynomolgus 4-1BB Ligand/TNFSF9 Protein 4258

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The 4-1BBL is the high affinity ligand of 4-1BB, also known as CD137L or TNFSF9. 4-1BB ligand (4-1BBL) is an inducible molecule present on several APC types, including B cells, macrophages and DCs.4-1BB:4-1BBL pathway seems to amplify the existing costimulatory signals, even if the engagement of 4-1BB in the presence of a strong TCR signaling can induce IL-2 production in a CD28-independent manner.
Protein names Tumor necrosis factor ligand superfamily member 9 (4-1BB ligand) (4-1BBL)
Gene names TNFSF9,TNFSF9
Protein family Tumor necrosis factor family
Mass 26625Da
Function FUNCTION: Cytokine that binds to TNFRSF9. Induces the proliferation of activated peripheral blood T-cells. May have a role in activation-induced cell death (AICD). May play a role in cognate interactions between T-cells and B-cells/macrophages. {ECO:0000269\|PubMed:20032458}.
Subellular location SUBCELLULAR LOCATION: Membrane; Single-pass type II membrane protein.
Tissues TISSUE SPECIFICITY: Expressed in brain, placenta, lung, skeletal muscle and kidney.
Structure SUBUNIT: Homotrimer. {ECO:0000269\|PubMed:20032458}.
Target Relevance information above includes information from UniProt accession: P41273
The UniProt Consortium

Data

ELISA with Cynomolgus 4-1BB Ligand/TNFSF9 Protein

Immobilized Cynomolgus/Rhesus macaque 4-1BB, His Tag at 0.2µg/ml (100µl/Well) on the plate. Dose response curve for Cynomolgus 4-1BB Ligand, hFc Tag with the EC50 of 3.3ng/ml determined by ELISA.

HPLC of Cynomolgus 4-1BB Ligand/TNFSF9 Protein

The purity of Cynomolgus 4-1BB Ligand is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Cynomolgus 4-1BB Ligand/TNFSF9 Protein

Cynomolgus 4-1BB Ligand on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.