SDS-PAGE gel of Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) 2065

Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer Protein 2065

$310.00 – $1,125.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and RMFPNAPYL peptide

SKU: 2065parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A140T913(Human HLA-A*02:01 α1&α2)&P01897(Mouse HLd α3)&&P01887(Mouse B2M)&RMFPNAPYL
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and RMFPNAPYL peptide of the WT-1.
molecular weight	The protein has a predicted MW of 48.04 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer Protein 2065

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The WT1 protein plays a role in cell growth, the process by which cells mature to perform specific functions (differentiation), and the self-destruction of cells (apoptosis). WT1 is differentially expressed in serous, endometrioid, clear cell, and mucinous carcinomas of the peritoneum, fallopian tube, ovary, and endometrium.The Human HLA-A0201 WT-1 (RMFPNAPYL) complex Protein is a complex of HLA-A0201 of Â the MHC Class I, B2M and RMFPNAPYL peptide of Â the WT-1.
Protein names Major histocompatibility complex, class I, A
Gene names HLA-A,HLA-A
Protein family MHC class I family
Mass 41449Da
Target Relevance information above includes information from UniProt accession: A0A140T913
The UniProt Consortium

ELISA with Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Mono

Immobilized Anti-HLA-A*02:01&B2M&WT-1 (RMFPNAPYL) Antibody, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer, His Tag with the EC50 of 39.6ng/ml determined by ELISA.

HPLC of Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer

The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL)

Chimeric HLA-A*02:01 (mα3) &mB2M&WT-1 (RMFPNAPYL) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.