SPR with Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monom 2060

ELISA with Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Mon 2060

HPLC of Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monome 2060

SDS-PAGE gel of Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA 2060

Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer Protein 2060

$310.00 – $1,125.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly25-Thr206(Human HLA-A*02:01 α1&α2) & Asp207-Glu299(Mouse H-2Ld α3), Ile21-Met119(mB2M) and YLEPGPVTA peptide

SKU: 2060parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
accession	A0A140T933(Human HLA-A*02:01 α1&α2)&P01897(Mouse HLd α3)&P01887(Mouse B2M)&YLEPGPVTA
express system	HEK293
product tag	C-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and YLEPGPVTA peptide of the gp100.
molecular weight	The protein has a predicted MW of 47.90 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer Protein 2060

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A0201 ofÂ the MHC Class I, B2M and YLEPGPVTA peptide ofÂ the gp100.
Protein names Major histocompatibility complex, class I, A
Gene names HLA-A,HLA-A
Protein family MHC class I family
Mass 34268Da
Target Relevance information above includes information from UniProt accession: A0A140T933
The UniProt Consortium

SPR with Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monom

Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag with an affinity constant of 67.84 nM as determined in SPR assay (Biacore T200).

ELISA with Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Mon

Immobilized Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 25.3ng/ml determined by ELISA (QC Test).

HPLC of Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monome

The purity of Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA

Chimeric HLA-A*02:01 (mα3) &mB2M&GP100 (YLEPGPVTA) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.