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Biotinylated Mouse PSMA/FOLH1 Protein (Primary Amine Labeling) 2756

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Pure: Yes (HPLC)
  • Amino Acid Range: Lys45-Ala752
SKU: 2756parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
express system

HEK293

product tag

N-His

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.

molecular weight

The protein has a predicted MW of 80.6 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Mouse PSMA/FOLH1 Protein (Primary Amine Labeling) 2756

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells.
Protein names
Glutamate carboxypeptidase 2 (EC 3.4.17.21) (Folate hydrolase 1) (Folylpoly-gamma-glutamate carboxypeptidase) (FGCP) (Glutamate carboxypeptidase II) (GCPII) (Membrane glutamate carboxypeptidase) (mGCP) (N-acetylated-alpha-linked acidic dipeptidase I) (NAALADase I) (Prostate-specific membrane antigen homolog) (Pteroylpoly-gamma-glutamate c
Gene names
Folh1,Folh1 Mopsm Naalad1
Protein family
Peptidase M28 family, M28B subfamily
Mass
10090Da
Function
Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides (By similarity). In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate.; Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC.
Catalytic activity
BINDING 212; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 259; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 271; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 274; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 379; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /ligand_note="catalytic"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 389; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /ligand_note="catalytic"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 389; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 426; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 427; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 435; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 438; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 455; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /ligand_note="catalytic"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 519..520; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 521; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 536..538; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 554..555; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 554; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 555; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 701..702; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"
Subellular location
Cell membrane ; Single-pass type II membrane protein .
Tissues
Expressed predominantly in the hippocampal region of the brain and in kidney. Lower levels in the ovary, testis and mandibular gland.
Structure
Homodimer.
Domain
Th
Target Relevance information above includes information from UniProt accession: O35409
The UniProt Consortium

HPLC of Biotinylated Mouse PSMA/FOLH1 Protein (Primary Amine Labeling)
The purity of Biotinylated Mouse PSMA is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Mouse PSMA/FOLH1 Protein (Primary Amine La
Biotinylated Mouse PSMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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