Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
express system | HEK293 |
product tag | N-His |
purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
background | Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells. |
molecular weight | The protein has a predicted MW of 80.6 kDa. Due to glycosylation, the protein migrates to 90-110 kDa based on Tris-Bis PAGE result. |
available size | 100 µg, 500 µg |
endotoxin | Less than 1EU per μg by the LAL method. |
Biotinylated Mouse PSMA/FOLH1 Protein (Primary Amine Labeling) 2756
$525.00 – $1,750.00
Summary
- Expression: HEK293
- Pure: Yes (HPLC)
- Amino Acid Range: Lys45-Ala752
Biotinylated Mouse PSMA/FOLH1 Protein (Primary Amine Labeling) 2756
protein |
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Size and concentration 100, 500µg and lyophilized |
Form Lyophilized |
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Storage buffer Shipped at ambient temperature. |
Purity > 95% as determined by Tris-Bis PAGE |
target relevance |
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Prostate-specific membrane antigen (PSMA) is an enzyme that in humans is encoded by the FOLH1 (folate hydrolase 1) gene, also known as Glutamate carboxypeptidase II (GCPII). Human PSMA is highly expressed in the prostate, roughly a hundred times greater than in most other tissues. In some prostate cancers, PSMA is the second-most upregulated gene product, with an 8- to 12-fold increase over levels in noncancerous prostate cells. |
Protein names Glutamate carboxypeptidase 2 (EC 3.4.17.21) (Folate hydrolase 1) (Folylpoly-gamma-glutamate carboxypeptidase) (FGCP) (Glutamate carboxypeptidase II) (GCPII) (Membrane glutamate carboxypeptidase) (mGCP) (N-acetylated-alpha-linked acidic dipeptidase I) (NAALADase I) (Prostate-specific membrane antigen homolog) (Pteroylpoly-gamma-glutamate c |
Gene names Folh1,Folh1 Mopsm Naalad1 |
Protein family Peptidase M28 family, M28B subfamily |
Mass 10090Da |
Function Has both folate hydrolase and N-acetylated-alpha-linked-acidic dipeptidase (NAALADase) activity. Has a preference for tri-alpha-glutamate peptides (By similarity). In the intestine, required for the uptake of folate. In the brain, modulates excitatory neurotransmission through the hydrolysis of the neuropeptide, N-aceylaspartylglutamate (NAAG), thereby releasing glutamate.; Also exhibits a dipeptidyl-peptidase IV type activity. In vitro, cleaves Gly-Pro-AMC. |
Catalytic activity BINDING 212; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 259; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 271; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 274; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 379; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /ligand_note="catalytic"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 389; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /ligand_note="catalytic"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 389; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 426; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 427; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 435; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 438; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 455; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /ligand_note="catalytic"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 519..520; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 521; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 536..538; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 554..555; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0"; BINDING 554; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 555; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /evidence="ECO:0000250|UniProtKB:Q04609"; BINDING 701..702; /ligand="substrate"; /evidence="ECO:0000250|UniProtKB:Q9Y3Q0" |
Subellular location Cell membrane ; Single-pass type II membrane protein . |
Tissues Expressed predominantly in the hippocampal region of the brain and in kidney. Lower levels in the ovary, testis and mandibular gland. |
Structure Homodimer. |
Domain Th |
Target Relevance information above includes information from UniProt accession: O35409 |
The UniProt Consortium |
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The purity of Biotinylated Mouse PSMA is greater than 95% as determined by SEC-HPLC. |
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Biotinylated Mouse PSMA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
Publications
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