SPR with Biotinylated Mouse IL-22 Protein 3353

ELISA with Biotinylated Mouse IL-22 Protein 3353

Biotinylated Mouse IL-22 Protein 3353

$525.00 – $1,750.00

Summary

Expression: E.coli
Functional: Yes (ELISA)
Amino Acid Range: Leu34-Val179

SKU: 3353parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	E.coli
product tag	N-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
background	The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
molecular weight	The protein has a predicted MW of 19.7 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Mouse IL-22 Protein 3353

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The cytokine interleukin-22 (IL-22) is a critical regulator of epithelial homeostasis. It has been implicated in multiple aspects of epithelial barrier function, including regulation of epithelial cell growth and permeability, production of mucus and antimicrobial proteins (AMPs), and complement production.
Protein names Interleukin-22 (IL-22) (IL-10-related T-cell-derived-inducible factor) (IL-TIF) (IL-TIF alpha) (Interleukin-22a) (IL-22a)
Gene names Il22,Il22 Il22a Iltif Iltifa
Protein family IL-10 family
Mass 10090Da
Function Cytokine that plays a critical role in modulating tissue responses during inflammation (PubMed:33852830, PubMed:35525330). Plays an essential role in the regeneration of epithelial cells to maintain barrier function after injury and for the prevention of further tissue damage (PubMed:33912578). Unlike most of the cytokines, has no effect on immune cells. Signals through a heterodimeric receptor composed of two subunits, the specific receptor IL22RA1 which is present on non-immune cells in many organs and the shared subunit IL10RB. Ligation of IL22RA1 with IL22 induces activation of the tyrosine kinases JAK1 and TYK2, which in turn activates STAT3. In turn, promotes cell survival and proliferation through STAT3, ERK1/2 and PI3K/AKT pathways. Promotes phosphorylation of GSK3B at 'Ser-9' and CTTN (PubMed:24742671). Promotes epithelial cell spreading (PubMed:24742671).
Subellular location Secreted.
Target Relevance information above includes information from UniProt accession: Q9JJY9
The UniProt Consortium

SPR with Biotinylated Mouse IL-22 Protein

Rat IL-22R alpha 1, His Tag immobilized on CM5 Chip can bind Biotinylated Mouse IL-22, His Tag with an affinity constant of 0.145 µM as determined in SPR assay (Biacore T200).

ELISA with Biotinylated Mouse IL-22 Protein

Immobilized Biotinylated Mouse IL-22, His Tag at 2µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Mouse IL-22R alpha 1, hFc Tag with the EC50 of 15.2ng/ml determined by ELISA (QC Test).

HPLC of Biotinylated Mouse IL-22 Protein

The purity of Biotinylated Mouse IL-22 is greater than 90% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Mouse IL-22 Protein

Biotinylated Mouse IL-22 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

1. **What is the concentration of the Biotinylated Mouse IL-22 Protein (3353)?**
- The concentration of the Biotinylated Mouse IL-22 Protein (3353) is provided at 0.1 mg/ml.

2. **How should this product be stored?**
- This product should be stored at -20°C. Avoid multiple freeze-thaw cycles to maintain optimal activity.

3. **Is the Biotinylated Mouse IL-22 Protein (3353) compatible with various experimental applications?**
- Yes, this protein is suitable for various applications such as ELISA, Western blot, and immunohistochemistry.

4. **Can this product be used for in vivo experiments?**
- This product is intended for in vitro research applications only and should not be used for in vivo experiments.

5. **What is the stability of the Biotinylated Mouse IL-22 Protein (3353)?**
- The stability of this protein is guaranteed for 12 months from the date of receipt when stored as directed at -20°C.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.