Biotinylated Mouse IL-2 Protein 3432

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ala21-Gln169

SKU: 3432parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	N-His-Avi
purity	> 95% as determined by Tris-Bis PAGE
background	Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15, 5 – 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.
molecular weight	The protein has a predicted MW of 20.1 kDa. Due to glycosylation, the protein migrates to 27-30 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per ug by the LAL method.

Biotinylated Mouse IL-2 Protein 3432

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin-2 (IL-2) is an interleukin, a type of cytokine signaling molecule in the immune system. It is a 15,5 - 16 kDa protein that regulates the activities of white blood cells (leukocytes, often lymphocytes) that are responsible for immunity. IL-2 has essential roles in key functions of the immune system, tolerance and immunity, primarily via its direct effects on T cells.
Protein names Interleukin-2 (IL-2) (T-cell growth factor) (TCGF)
Protein family 320
Function Cytokine produced by activated CD4-positive helper T-cells and to a lesser extend activated CD8-positive T-cells and natural killer (NK) cells that plays pivotal roles in the immune response and tolerance (PubMed:14614860, PubMed:9814585). Binds to a receptor complex composed of either the high-affinity trimeric IL-2R (IL2RA/CD25, IL2RB/CD122 and IL2RG/CD132) or the low-affinity dimeric IL-2R (IL2RB and IL2RG). Interaction with the receptor leads to oligomerization and conformation changes in the IL-2R subunits resulting in downstream signaling starting with phosphorylation of JAK1 and JAK3. In turn, JAK1 and JAK3 phosphorylate the receptor to form a docking site leading to the phosphorylation of several substrates including STAT5 (PubMed:14614860, PubMed:27018889). This process leads to activation of several pathways including STAT, phosphoinositide-3-kinase/PI3K and mitogen-activated protein kinase/MAPK pathways. Functions as a T-cell growth factor and can increase NK-cell cytolytic activity as well. Promotes strong proliferation of activated B-cells and subsequently immunoglobulin production. Plays a pivotal role in regulating the adaptive immune system by controlling the survival and proliferation of regulatory T-cells, which are required for the maintenance of immune tolerance (PubMed:14614860). Moreover, participates in the differentiation and homeostasis of effector T-cell subsets, including Th1, Th2, Th17 as well as memory CD8-positive T-cells (PubMed:9814585).
Pathway 214
Subellular location Secreted.
Tissues Produced by immune cells including dendritic cells. In contrast, macrophages do not produce IL2 upon bacterial stimulation.
Target Relevance information above includes information from UniProt accession: P04351
The UniProt Consortium

ELISA with Biotinylated Mouse IL-2 Protein

Immobilized Mouse IL-2 R alpha, His Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Mouse IL-2, His Tag with the EC50 of 0.21µg/ml determined by ELISA.

SDS-PAGE gel of Biotinylated Mouse IL-2 Protein

Biotinylated Mouse IL-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

1. What is the concentration of the Biotinylated Mouse IL-2 Protein (3432)?
Answer: The concentration of the Biotinylated Mouse IL-2 Protein (3432) is 10 µg/ml.

2. How should I store this product?
Answer: To maintain the stability and activity of the Biotinylated Mouse IL-2 Protein (3432), it should be stored at -20°C upon arrival in a manual defrost freezer.

3. Can this biotinylated protein be used for flow cytometry applications?
Answer: Yes, the Biotinylated Mouse IL-2 Protein (3432) is suitable for flow cytometry applications, including cell staining and analysis.

4. Is this biotinylated protein compatible with commonly used detection systems?
Answer: Yes, the Biotinylated Mouse IL-2 Protein (3432) is compatible with various detection systems that utilize streptavidin conjugates for signal amplification.

5. What is the recommended working concentration for using this biotinylated protein in assays?
Answer: The recommended working concentration of the Biotinylated Mouse IL-2 Protein (3432) in assays is typically in the range of 1-10 µg/ml, but optimization may be required based on the specific application.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.