ELISA with Biotinylated Human ULBP-2 Protein 4780

Binding assay with Biotinylated Human ULBP-2 Protein 4780

Biotinylated Human ULBP-2 Protein 4780

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (binding assay)
Amino Acid Range: Gly26-Ser217

SKU: 4780parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	ULBP2 expression was however linked to expression of mature CD57() NK cells. In particular, expression of ULBP2 was strongest on those NK cells that had evidence of recent activation and proliferation. We suggest that ULBP2 could be used to identify recently activated "mature" NK cells.
molecular weight	The protein has a predicted MW of 24.6 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human ULBP-2 Protein 4780

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
ULBP2 expression was however linked to expression of mature CD57() NK cells. In particular, expression of ULBP2 was strongest on those NK cells that had evidence of recent activation and proliferation. We suggest that ULBP2 could be used to identify recently activated "mature" NK cells.
Protein names UL16-binding protein 2 (ALCAN-alpha) (NKG2D ligand 2) (N2DL-2) (NKG2DL2) (Retinoic acid early transcript 1H)
Gene names ULBP2,ULBP2 N2DL2 RAET1H UNQ463/PRO791
Protein family MHC class I family
Mass 9606Da
Function Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity.
Subellular location Cell membrane ; Lipid-anchor, GPI-anchor. Endoplasmic reticulum. Secreted. Note=In CMV-infected fibroblasts, detected in the endoplasmic reticulum/cis-Golgi.
Tissues Expressed in various types of cancer cell lines and in the fetus, but not in normal tissues.
Structure Interacts with KLRK1/NKG2D (PubMed:11777960). Does not bind to beta2-microglobulin (PubMed:12782710).; (Microbial infection) In CMV-infected cells, interacts with the viral glycoprotein UL16; this interaction causes ULBP2 retention in the endoplasmic reticulum and cis-Golgi and prevents binding to and activation of KLRK1/NKG2D, providing CMV with an immune evasion mechanism.
Target Relevance information above includes information from UniProt accession: Q9BZM5
The UniProt Consortium

Data

ELISA with Biotinylated Human ULBP-2 Protein

Immobilized Biotinylated Human ULBP-2, His Tag at 1µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Human NKG2D, hFc Tag with the EC50 of 0.25µg/ml determined by ELISA.

Binding assay with Biotinylated Human ULBP-2 Protein

Serial dilutions of Anti-NKG2D Antibody, hFc Tag were added into Biotinylated Human ULBP-2, His Tag : Human NKG2D, hFc Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 45.2ng/ml.

HPLC of Biotinylated Human ULBP-2 Protein

The purity of Biotinylated Human ULBP-2 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human ULBP-2 Protein

Biotinylated Human ULBP-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.