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Biotinylated Human ULBP-1 Protein 4722

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gly26-Pro215
SKU: 4722parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
product tag

C-His-Avi, also biotinylated

Biotinylated Human ULBP-1 Protein 4722

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Cell surface proteins major histocompatibility complex (MHC) class I-related chain A (MICA) and UL16-binding proteins (ULBP) 1, 2, and 3 are up-regulated upon infection or tumor transformation and can activate human natural killer (NK) cells.
Protein names
UL16-binding protein 1 (ALCAN-beta) (NKG2D ligand 1) (N2DL-1) (NKG2DL1) (Retinoic acid early transcript 1I)
Gene names
ULBP1,ULBP1 N2DL1 RAET1I
Protein family
MHC class I family
Mass
9606Da
Function
FUNCTION: Binds and activates the KLRK1/NKG2D receptor, mediating natural killer cell cytotoxicity. {ECO:0000269|PubMed:11777960}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:12782710}; Lipid-anchor, GPI-anchor {ECO:0000269|PubMed:12782710}. Endoplasmic reticulum {ECO:0000269|PubMed:12782710}. Note=In CMV-infected fibroblasts, detected in the endoplasmic reticulum/cis-Golgi. {ECO:0000269|PubMed:12782710}.
Tissues
TISSUE SPECIFICITY: Expressed in T-cells, B-cells, erythroleukemia cell lines and in a wide range of tissues including heart, brain, lung, liver, testis, lymph node, thymus, tonsil and bone marrow. Also found in fetal heart, brain, lung and liver.
Structure
SUBUNIT: Interacts with KLRK1/NKG2D (PubMed:11777960). Does not bind to beta2-microglobulin (PubMed:12782710). {ECO:0000269|PubMed:11777960, ECO:0000269|PubMed:12782710}.; SUBUNIT: (Microbial infection) In CMV-infected cells, interacts with the viral glycoprotein UL16; this interaction causes ULBP1 retention in the endoplasmic reticulum and cis-Golgi and prevents binding to and activation of KLRK1/NKG2D, providing CMV with an immune evasion mechanism. {ECO:0000269|PubMed:12782710}.
Target Relevance information above includes information from UniProt accession : Q9BZM6
The UniProt Consortium

Data

ELISA with Biotinylated Human ULBP-1 Protein
Immobilized Human NKG2D, hFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human ULBP-1, His Tag with the EC50 of 0.14µg/ml determined by ELISA.
HPLC of Biotinylated Human ULBP-1 Protein
The purity of Biotinylated Human ULBP-1 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human ULBP-1 Protein
Biotinylated Human ULBP-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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