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Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein 4831

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Pro34-Asn239
Weight1 lbs
Dimensions9 × 5 × 2 in
product tag

C-His-Avi, also biotinylated

Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein 4831

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Tumor necrosis factor receptor superfamily member 10A (TNFRSF10A) is also known as TNF-related apoptosis-inducing ligand receptor 1 (TRAIL-R1), Death receptor 4 (DR4), CD261 and APO2, which belongs to TNF superfamily.The expression of apoptosis-inducing TRAIL-R1 and TRAIL-R2 and of the decoy receptors TRAIL-R3 and TRAIL-R4 was systematically studied in all developmental stages of peripheral B cells isolated from the blood and secondary lymphoid organs. Expression of TRAIL-Rs is modulated along development, with highest levels observed in germinal center B cells.
Protein names
Tumor necrosis factor receptor superfamily member 10A (Death receptor 4) (TNF-related apoptosis-inducing ligand receptor 1) (TRAIL receptor 1) (TRAIL-R1) (CD antigen CD261)
Gene names
TNFRSF10A,TNFRSF10A APO2 DR4 TRAILR1
Mass
50089Da
Function
FUNCTION: Receptor for the cytotoxic ligand TNFSF10/TRAIL (PubMed:26457518). The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis (PubMed:19090789). Promotes the activation of NF-kappa-B (PubMed:9430227). {ECO:0000269|PubMed:19090789, ECO:0000269|PubMed:9430227}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:22240897}; Single-pass type I membrane protein {ECO:0000255}. Membrane raft {ECO:0000269|PubMed:19090789, ECO:0000269|PubMed:22240897}. Cytoplasm, cytosol {ECO:0000269|PubMed:22240897}. Note=Palmitoylation is required for association with membranes. {ECO:0000269|PubMed:22240897}.
Tissues
TISSUE SPECIFICITY: Widely expressed. High levels are found in spleen, peripheral blood leukocytes, small intestine and thymus, but also in K-562 erythroleukemia cells, MCF-7 breast carcinoma cells and activated T-cells.
Structure
SUBUNIT: Monomer (PubMed:26457518). Homooligomers and heterooligomers with TNFRSF10B (PubMed:19090789). Three TNFRSF10A molecules interact with the TNFSF10 homotrimer (PubMed:26457518). Can interact with TRADD and RIPK1. Interacts with ARAP1. In the absence of stimulation, interacts with BIRC2, DDX3X and GSK3B. The interaction with BIRC2 and DDX3X is further enhanced upon receptor stimulation and accompanied by DDX3X and BIRC2 cleavage (PubMed:18846110). Interacts with ZDHHC3 (PubMed:22240897). {ECO:0000269|PubMed:18165900, ECO:0000269|PubMed:18846110, ECO:0000269|PubMed:19090789, ECO:0000269|PubMed:22240897, ECO:0000269|PubMed:26457518}.; SUBUNIT: (Microbial infection) Interacts with HCMV protein UL141; this interaction prevents TNFRSF10A cell surface expression. {ECO:0000269|PubMed:23498957}.
Post-translational modification
PTM: Palmitoylated (PubMed:19090789). Palmitoylation of TNFRSF10A is required for its association with lipid rafts, oligomerization and function in TRAIL-induced cell death (PubMed:19090789). Palmitoylated by ZDHHC3 (Probable). {ECO:0000269|PubMed:19090789, ECO:0000305|PubMed:22240897}.
Domain
TOPO_DOM 2
Target Relevance information above includes information from UniProt accession : O00220
The UniProt Consortium

Data

SPR with Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein
Biotinylated Human TRAIL R1, His-Avi Tag immobilized on CM5 Chip can bind Human TRAIL, No Tag with an affinity constant of 0.315 nM as determined in SPR assay (Biacore T200).
ELISA with Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein
Immobilized Human TRAIL, No Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human TRAIL R1, His Tag with the EC50 of 0.31µg/ml determined by ELISA (QC Test).
HPLC of Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein
The purity of Biotinylated Human TRAIL R1 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human TRAIL R1/DR4/TNFRSF10A Protein
Biotinylated Human TRAIL R1 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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