ELISA with Biotinylated Human TPBG/5T4 Protein 4381

Biotinylated Human TPBG/5T4 Protein 4381

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ser32-Ser355

SKU: 4381parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
molecular weight	The protein has a predicted MW of 38.2 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human TPBG/5T4 Protein 4381

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The oncofetal tumor-associated antigen 5T4 (TBGP) has been linked with CSC properties in several different malignancies. 5T4 has functional attributes that are relevant to the spread of tumors including through EMT, CXCR4/CXCL12, Wnt, and Hippo pathways which may all contribute through the mobilization of CSCs.
Protein names Trophoblast glycoprotein (5T4 oncofetal antigen) (5T4 oncofetal trophoblast glycoprotein) (5T4 oncotrophoblast glycoprotein) (M6P1) (Wnt-activated inhibitory factor 1) (WAIF1)
Gene names TPBG,TPBG 5T4
Mass 9606Da
Function May function as an inhibitor of Wnt/beta-catenin signaling by indirectly interacting with LRP6 and blocking Wnt3a-dependent LRP6 internalization.
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
Post-translational modification Highly glycosylated.
Domain Th
Target Relevance information above includes information from UniProt accession: Q13641
The UniProt Consortium

Data

ELISA with Biotinylated Human TPBG/5T4 Protein

Immobilized Biotinylated Human TPBG, His Tag at 0.5µg/ml (100µl/Well) on streptavidin (5µg/ml) precoated plate. Dose response curve for Anti-TPBG Antibody, hFc Tag with the EC50 of 8.7ng/ml determined by ELISA.

HPLC of Biotinylated Human TPBG/5T4 Protein

The purity of Biotinylated Human TPBG is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human TPBG/5T4 Protein

Biotinylated Human TPBG on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.