ELISA with Biotinylated Human TIGIT Protein 5203

Biotinylated Human TIGIT Protein 5203

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Met22-Pro141

SKU: 5203parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.TIGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
molecular weight	The protein has a predicted MW of 16.1 kDa. Due to glycosylation, the protein migrates to 20-25 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human TIGIT Protein 5203

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
TIGIT, also called Vstm3, Vsig9, and WUCAM, is a transmembrane protein in the CD28 family of the Ig superfamily proteins.TIGIT is expressed at low levels on peripheral memory and regulatory CD4 T-cells and NK cells and is up-regulated following activation of these cells.
Protein names T-cell immunoreceptor with Ig and ITIM domains (V-set and immunoglobulin domain-containing protein 9) (V-set and transmembrane domain-containing protein 3)
Gene names TIGIT,TIGIT VSIG9 VSTM3
Mass 9606Da
Function Binds with high affinity to the poliovirus receptor (PVR) which causes increased secretion of IL10 and decreased secretion of IL12B and suppresses T-cell activation by promoting the generation of mature immunoregulatory dendritic cells.
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Expressed at low levels on peripheral memory and regulatory CD4+ T-cells and NK cells and is up-regulated following activation of these cells (at protein level).
Structure Homodimer in cis; binds with high affinity to PVR, forming a heterotetrameric assembly of two TIGIT and two PVR molecules. Binds with lower affinity to NECTIN2 and NECTIN3.
Domain Co
Target Relevance information above includes information from UniProt accession: Q495A1
The UniProt Consortium

Data

ELISA with Biotinylated Human TIGIT Protein

Immobilized Biotinylated Human TIGIT, His-Avi Tag at 0.5µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Human CD155, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.

HPLC of Biotinylated Human TIGIT Protein

The purity of Biotinylated Human TIGIT is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human TIGIT Protein

Biotinylated Human TIGIT on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.