ELISA with Biotinylated Human SIRP alpha V3 Protein 3509

Biotinylated Human SIRP alpha V3 Protein 3509

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu31-Arg369(H54L)

SKU: 3509parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Signal regulatory protein α (SIRPα) is a regulatory membrane glycoprotein from SIRP family expressed mainly by myeloid cells and also by stem cells or neurons.SIRPα acts as inhibitory receptor and interacts with a broadly expressed transmembrane protein CD47 also called the "don´t eat me" signal.Cancer cells highly expressed CD47 that activate SIRP α and inhibit macrophage-mediated destruction.
molecular weight	The protein has a predicted MW of 39.8 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human SIRP alpha V3 Protein 3509

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

No information

target relevance

Data

ELISA with Biotinylated Human SIRP alpha V3 Protein

Immobilized Biotinylated Human SIRP alpha V3, His Tag at 1µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Human CD47, hFc Tag with the EC50 of 11.3ng/ml determined by ELISA.

HPLC of Biotinylated Human SIRP alpha V3 Protein

The purity of Biotinylated Human SIRP alpha V3 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human SIRP alpha V3 Protein

Biotinylated Human SIRP alpha V3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.