Weight | 1 lbs |
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Dimensions | 9 × 5 × 2 in |
express system | HEK293 |
product tag | C-His-Avi |
purity | > 95% as determined by Tris-Bis PAGE |
background | Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains.Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2, 3-linked sialic acid. Also binds to alpha-2, 6-linked sialic acid. |
molecular weight | The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result. |
available size | 100 µg, 500 µg |
endotoxin | Less than 1EU per μg by the LAL method. |
Biotinylated Human Siglec-8 Protein 4787
$525.00 – $1,750.00
Summary
- Expression: HEK293
- Pure: Yes (SDS-PAGE)
- Amino Acid Range: Met17-Ala363
Biotinylated Human Siglec-8 Protein 4787
protein |
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Size and concentration 100, 500µg and lyophilized |
Form Lyophilized |
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Storage buffer Shipped at ambient temperature. |
Purity > 95% as determined by Tris-Bis PAGE |
target relevance |
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Siglec-8, also known as SAF, is an approximately 75 kDa transmembrane glycoprotein in the Siglec family of sialic acid-binding immune regulatory molecules. Mature human Siglec-8 consists of a 347 amino acid (aa) extracellular domain (ECD) with three Ig-like domains.Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells. Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. |
Protein names Sialic acid-binding Ig-like lectin 8 (Siglec-8) (Sialoadhesin family member 2) (SAF-2) |
Gene names SIGLEC8,SIGLEC8 SAF2 |
Protein family Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family |
Mass 9606Da |
Function Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells (PubMed:10625619, PubMed:10856141). Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619). Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes (PubMed:27357658). |
Catalytic activity BINDING 23; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269|PubMed:27357658, ECO:0007744|PDB:2N7B"; BINDING 72..75; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269|PubMed:27357658, ECO:0007744|PDB:2N7B"; BINDING 125; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269|PubMed:27357658, ECO:0007744|PDB:2N7B"; BINDING 134..138; /ligand="a carbohydrate"; /ligand_id="ChEBI:CHEBI:16646"; /evidence="ECO:0000269|PubMed:27357658, ECO:0007744|PDB:2N7B" |
Subellular location Membrane; Single-pass type I membrane protein. |
Tissues Expressed specifically on red blood cells namely basophil, mast cells and eosinophils. |
Domain Co |
Target Relevance information above includes information from UniProt accession: Q9NYZ4 |
The UniProt Consortium |
Data
Biotinylated Human Siglec-8 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
Publications
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.pmid | title | authors | citation |
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Protocols
relevant to this product |
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Documents
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