ELISA with Biotinylated Human Siglec-10 (R119A) Protein 3372

Biotinylated Human Siglec-10 (R119A) Protein 3372

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Met17-Thr546

SKU: 3372parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
molecular weight	The protein has a predicted MW of 62.64 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per ug by the LAL method.

Biotinylated Human Siglec-10 (R119A) Protein 3372

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Siglec-10 is a ligand for CD52, the target of the therapeutic monoclonal antibody Alemtuzumab. It is also reported to bind to Vascular adhesion protein 1 (VAP-1) and to the co-stimulatory molecule CD24 also known as HSA (Heat-stable antigen).Siglecs (sialic acid binding Ig-like lectins) are I-type lectins that belong to the immunoglobulin superfamily. They are characterized by an N-terminal Ig-like V-type domain which mediates sialic acid binding, followed by a varying number of Ig-like C2-type domains. Siglecs 5-11 constitute the CD33/Siglec-3 related group, and are differentially expressed in the hematopoietic system.
Protein names Sialic acid-binding Ig-like lectin 10 (Siglec-10) (Siglec-like protein 2)
Gene names SIGLEC10,SIGLEC10 SLG2 UNQ477/PRO940
Protein family Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
Mass 9606Da
Function Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- or alpha-2,6-linked sialic acid (By similarity). The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. In the immune response, seems to act as an inhibitory receptor upon ligand induced tyrosine phosphorylation by recruiting cytoplasmic phosphatase(s) via their SH2 domain(s) that block signal transduction through dephosphorylation of signaling molecules (PubMed:11284738, PubMed:12163025). Involved in negative regulation of B-cell antigen receptor signaling. The inhibition of B cell activation is dependent on PTPN6/SHP-1 (By similarity). In association with CD24 may be involved in the selective suppression of the immune response to danger-associated molecular patterns (DAMPs) such as HMGB1, HSP70 and HSP90 (By similarity). In association with CD24 may regulate the immune repsonse of natural killer (NK) cells (PubMed:25450598). Plays a role in the control of autoimmunity (By similarity). During initiation of adaptive immune responses by CD8-alpha(+) dendritic cells inhibits cross-presentation by impairing the formation of MHC class I-peptide complexes. The function seems to implicate recruitment of PTPN6/SHP-1, which dephosphorylates NCF1 of the NADPH oxidase complex consequently promoting phagosomal acidification (By similarity).
Subellular location [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Cell membrane; Single-pass type I membrane protein.; [Isoform 3]: Cell membrane; Single-pass type I membrane protein.; [Isoform 4]: Cell membrane; Single-pass type I membrane protein.; [Isoform 5]: Secreted.
Tissues Expressed by peripheral blood leukocytes (eosinophils, monocytes and a natural killer cell subpopulation). Isoform 5 is found to be the most abundant isoform. Found in lymph node, lung, ovary and appendix. Isoform 1 is found at high levels and isoform 2 at lower levels in bone marrow, spleen and spinal chord. Isoform 2 is also found in brain. Isoform 4 is specifically found in natural killer cells.
Structure Interacts with PTPN6/SHP-1 upon phosphorylation (PubMed:12163025). Interacts with NCF1 (By similarity). Interacts with CD24; the probable CD24:SIGLEC10 complex is proposed to inhibit HGMB1-mediated tissue damage immune response. Interacts with HMGB1; the interaction is dependent on CD24 (PubMed:19264983). Interacts with RIGI, CBL and PTPN11 (By similarity).
Post-translational modification Phosphorylation of Tyr-667 is involved in binding to PTPN6.
Domain Co
Target Relevance information above includes information from UniProt accession: Q96LC7
The UniProt Consortium

ELISA with Biotinylated Human Siglec-10 (R119A) Protein

Immobilized Biotinylated Human Siglec-10 (R119A) , His Tag at 2µg/ml (100µl/Well) on the streptavidin (5µg/ml) precoated plate. Dose response curve for Anti-Siglec-10 Antibody, hFc Tag with the EC50 of 9.1ng/ml determined by ELISA.

HPLC of Biotinylated Human Siglec-10 (R119A) Protein

The purity of Biotinylated Human Siglec-10 (R119A) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human Siglec-10 (R119A) Protein

Biotinylated Human Siglec-10 (R119A) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

1. Question: What is the size and purity of the Biotinylated Human Siglec-10 R119A Protein (3372)?
Answer: The Biotinylated Human Siglec-10 R119A Protein (3372) is available in 10 µg size with a purity of over 90%.

2. Question: How should I store the Biotinylated Human Siglec-10 R119A Protein (3372) for long-term use?
Answer: It is recommended to store the protein at -20°C upon arrival. For long-term storage, aliquot the protein and store at -80°C to prevent frequent freeze-thaw cycles.

3. Question: Is the Biotinylated Human Siglec-10 R119A Protein (3372) suitable for use in flow cytometry applications?
Answer: Yes, this protein is specifically designed and validated for applications such as flow cytometry, making it suitable for cell surface protein detection and analysis.

4. Question: Can I use the Biotinylated Human Siglec-10 R119A Protein (3372) in research involving protein-protein interactions?
Answer: Yes, this protein is suitable for studying protein-protein interactions, receptor-binding studies, and other functional assays in the context of Siglec-10 research.

5. Question: Is the Biotinylated Human Siglec-10 R119A Protein (3372) compatible with commonly used detection methods and reagents?
Answer: Yes, the biotinylated format of this protein allows for easy conjugation with streptavidin-based detection systems commonly used in immunoassays and other protein detection methods.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.