ELISA with Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer?Pro 2412

HPLC of Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer?Protei 2412

SDS-PAGE gel of Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monome 2412

Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer Protein 2412

$310.00 – $1,250.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly25-Thr305(HLA-A*02:01) & Ile21-Met119(B2M)

SKU: 2412parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	HLA-A02:01&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
molecular weight	The protein has a predicted MW of 48.10 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer Protein 2412

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
HLA-A02:01&B2M&Peptide ready Monomer is absent from peptide, namely peptide-receptive MHC. It can be loaded with antigenic peptides matching HLA-A02:01. Peptide ready MHC molecules comprising human HLA alleles and B2M, which can be readily tetramerized and loaded with peptides of choice in a high-throughput manner.
Protein names Major histocompatibility complex, class I, A
Gene names HLA-A,HLA-A
Protein family MHC class I family
Mass 41449Da
Target Relevance information above includes information from UniProt accession: A0A140T913
The UniProt Consortium

ELISA with Biotinylated Human Peptide Ready HLA-A*02:01&B2M monomer~-Pro

Immobilized Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer, His Tag at 1µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-HLA class I (W6/32) Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.

HPLC of Biotinylated Human Peptide Ready HLA-A*02:01&B2M monomer~-Protei

The purity of Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monome

Biotinylated Human Peptide Ready HLA-A*02:01&B2M Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.