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Biotinylated Human P-Selectin/CD62P Protein 4676

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Trp42-Ala771
Weight1 lbs
Dimensions9 × 5 × 2 in
product tag

C-His-Avi, also biotinylated

Biotinylated Human P-Selectin/CD62P Protein 4676

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Human P-Selectin (GMP-140, LECAM-3, PADGEM, CD62P), a member of the Selectin family, is a cell surface glycoprotein expressed by activated platelets and endothelial cells. A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation.
Protein names
P-selectin (CD62 antigen-like family member P) (Granule membrane protein 140) (GMP-140) (Leukocyte-endothelial cell adhesion molecule 3) (LECAM3) (Platelet activation dependent granule-external membrane protein) (PADGEM) (CD antigen CD62P)
Gene names
SELP,SELP GMRP GRMP
Protein family
Selectin/LECAM family
Mass
90820Da
Function
FUNCTION: Ca(2+)-dependent receptor for myeloid cells that binds to carbohydrates on neutrophils and monocytes. Mediates the interaction of activated endothelial cells or platelets with leukocytes. The ligand recognized is sialyl-Lewis X. Mediates rapid rolling of leukocyte rolling over vascular surfaces during the initial steps in inflammation through interaction with SELPLG. {ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:28011641, ECO:0000269|PubMed:7585950}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:28011641}; Single-pass type I membrane protein {ECO:0000305|PubMed:28011641}.
Tissues
TISSUE SPECIFICITY: Stored in the alpha-granules of platelets and Weibel-Palade bodies of endothelial cells. Upon cell activation by agonists, P-selectin is transported rapidly to the cell surface.
Structure
SUBUNIT: Interacts with SNX17. Interacts with SELPLG/PSGL1 and PODXL2 and mediates neutrophil adhesion and leukocyte rolling. This interaction requires the sialyl-Lewis X epitope of SELPLG and PODXL2, and specific tyrosine sulfation on SELPLG. {ECO:0000269|PubMed:11081633, ECO:0000269|PubMed:11237770, ECO:0000269|PubMed:15769472, ECO:0000269|PubMed:18606703, ECO:0000269|PubMed:7559387, ECO:0000269|PubMed:7585949, ECO:0000269|PubMed:7585950}.
Domain
TOPO_DOM 4
Target Relevance information above includes information from UniProt accession : P16109
The UniProt Consortium

Data

ELISA with Biotinylated Human P-Selectin/CD62P Protein
Immobilized Biotinylated Human P-Selectin, His Tag at 1µg/ml (100µl/Well) on streptavidin (5µg/ml) precoated plate. Dose response curve for Anti-P-Selectin Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
HPLC of Biotinylated Human P-Selectin/CD62P Protein
The purity of Biotinylated Human P-Selectin is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human P-Selectin/CD62P Protein
Biotinylated Human P-Selectin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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