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Biotinylated Human NKG2D/CD314 Protein 4696

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Phe78-Val216
SKU: 4696parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
product tag

N-His-Avi, also biotinylated

Biotinylated Human NKG2D/CD314 Protein 4696

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
NKG2D is a type II transmembrane glycoprotein having an extracellular lectin-like domain. This domain lacks the recognizable calcium-binding sites found in true C-type lectins and binds protein rather than carbohydrate ligands. Human NKG2D is expressed on CD8 alpha beta T cells, gamma δ T cells, NK cells and NKT cells.
Protein names
NKG2-D type II integral membrane protein (Killer cell lectin-like receptor subfamily K member 1) (NK cell receptor D) (NKG2-D-activating NK receptor) (CD antigen CD314)
Gene names
KLRK1,KLRK1 D12S2489E NKG2D
Mass
9606Da
Function
FUNCTION: Functions as an activating and costimulatory receptor involved in immunosurveillance upon binding to various cellular stress-inducible ligands displayed at the surface of autologous tumor cells and virus-infected cells. Provides both stimulatory and costimulatory innate immune responses on activated killer (NK) cells, leading to cytotoxic activity. Acts as a costimulatory receptor for T-cell receptor (TCR) in CD8(+) T-cell-mediated adaptive immune responses by amplifying T-cell activation. Stimulates perforin-mediated elimination of ligand-expressing tumor cells. Signaling involves calcium influx, culminating in the expression of TNF-alpha. Participates in NK cell-mediated bone marrow graft rejection. May play a regulatory role in differentiation and survival of NK cells. Binds to ligands belonging to various subfamilies of MHC class I-related glycoproteins including MICA, MICB, RAET1E, RAET1G, RAET1L/ULBP6, ULBP1, ULBP2, ULBP3 (ULBP2>ULBP1>ULBP3) and ULBP4. {ECO:0000269|PubMed:10426994, ECO:0000269|PubMed:11224526, ECO:0000269|PubMed:11777960, ECO:0000269|PubMed:15240696, ECO:0000269|PubMed:19658097, ECO:0000269|PubMed:21898152, ECO:0000269|PubMed:23298206, ECO:0000269|PubMed:28559451}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:10426994, ECO:0000269|PubMed:11015446, ECO:0000269|PubMed:15294961}; Single-pass type II membrane protein {ECO:0000269|PubMed:10426994, ECO:0000269|PubMed:11015446, ECO:0000269|PubMed:15294961}. Note=Colocalized with HCST on the cell surface.
Tissues
TISSUE SPECIFICITY: Expressed in natural killer (NK) cells, CD8(+) alpha-beta and gamma-delta T-cells. Expressed on essentially all CD56+CD3- NK cells from freshly isolated PBMC. Expressed in interferon-producing killer dendritic cells (IKDCs). {ECO:0000269|PubMed:10426993, ECO:0000269|PubMed:15294961, ECO:0000269|PubMed:16444266}.
Structure
SUBUNIT: Homodimer; disulfide-linked. Heterohexamer composed of two subunits of KLRK1 and four subunits of HCST/DAP10. Interacts (via transmembrane domain) with HCST/DAP10 (via transmembrane domain); the interaction is required for KLRK1 NK cell surface and induces NK cell-mediated cytotoxicity. Does not interact with TYROBP. Interacts with CEACAM1; recruits PTPN6 that dephosphorylates VAV1 (PubMed:23696226). {ECO:0000269|PubMed:10426993, ECO:0000269|PubMed:10426994, ECO:0000269|PubMed:11015446, ECO:0000269|PubMed:11754823, ECO:0000269|PubMed:15294961, ECO:0000269|PubMed:15894612, ECO:0000269|PubMed:23696226}.
Target Relevance information above includes information from UniProt accession : P26718
The UniProt Consortium

Data

ELISA with Biotinylated Human NKG2D/CD314 Protein
Immobilized Anti-NKG2D Antibody at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human NKG2D, His Tag with the EC50 of 0.39µg/ml determined by ELISA.
HPLC of Biotinylated Human NKG2D/CD314 Protein
The purity of Biotinylated Human NKG2D is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human NKG2D/CD314 Protein
Biotinylated Human NKG2D on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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