ELISA with Biotinylated Human MSLN/Mesothelin Protein 4683

Activity assay with Biotinylated Human MSLN/Mesothelin Protein 4683

Biotinylated Human MSLN/Mesothelin Protein 4683

$525.00 – $1,750.00

Summary

Expression: HEK293
Active: Yes (catalytic)
Amino Acid Range: Glu296-Gly580

SKU: 4683parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-hFc-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
molecular weight	The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human MSLN/Mesothelin Protein 4683

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
Protein names Mesothelin (CAK1 antigen) (Pre-pro-megakaryocyte-potentiating factor) [Cleaved into: Megakaryocyte-potentiating factor (MPF); Mesothelin, cleaved form]
Gene names MSLN,MSLN MPF
Protein family Mesothelin family
Mass 9606Da
Function Membrane-anchored forms may play a role in cellular adhesion.; Megakaryocyte-potentiating factor (MPF) potentiates megakaryocyte colony formation in vitro.
Subellular location Cell membrane; Lipid-anchor, GPI-anchor. Golgi apparatus.; [Megakaryocyte-potentiating factor]: Secreted.; [Isoform 3]: Secreted.
Tissues Expressed in lung. Expressed at low levels in heart, placenta and kidney. Expressed in mesothelial cells. Highly expressed in mesotheliomas, ovarian cancers, and some squamous cell carcinomas (at protein level).
Structure Interacts with MUC16.
Post-translational modification Both MPF and the cleaved form of mesothelin are N-glycosylated.; Proteolytically cleaved by a furin-like convertase to generate megakaryocyte-potentiating factor (MPF), and the cleaved form of mesothelin.
Target Relevance information above includes information from UniProt accession: Q13421
The UniProt Consortium

Data

ELISA with Biotinylated Human MSLN/Mesothelin Protein

Immobilized Human CA125, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 44.1ng/ml determined by ELISA (QC Test).

Immobilized Anti-MSLN Antibody, hFc Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human MSLN, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA.

Activity assay with Biotinylated Human MSLN/Mesothelin Protein

Use Biotinylated Human MSLN-hFc-Avi protein to detect the expression rate of Anti-MSLN-CAR positive cell. 293T cells and anti-MSLN CAR-293T cells were incubated with Biotinylated human MSLN-hFc-Avi Tag. Non-transfected 293T cells and Fc-labeled protein control were used as negative control. SA-FITC was used to evaluate the binding activity of Biotinylated Human MSLN.

HPLC of Biotinylated Human MSLN/Mesothelin Protein

The purity of Biotinylated Human MSLN is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human MSLN/Mesothelin Protein

Biotinylated Human MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.