Skip to content

Biotinylated Human MICB Protein 4776

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Ala23-Gly298
SKU: 4776parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
product tag

C-His-Avi, also biotinylated

Biotinylated Human MICB Protein 4776

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
MICB (MHC class I chain-related gene B) is a transmembrane glycoprotein that functions as a ligand for NKG2D. A closely related protein, MICA, shares 85% amino acid identity with MICB.MICB Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis.
Protein names
MHC class I polypeptide-related sequence B (MIC-B)
Gene names
MICB,MICB PERB11.2
Protein family
MHC class I family, MIC subfamily
Mass
9606Da
Function
FUNCTION: Seems to have no role in antigen presentation. Acts as a stress-induced self-antigen that is recognized by gamma delta T cells. Ligand for the KLRK1/NKG2D receptor. Binding to KLRK1 leads to cell lysis. {ECO:0000269|PubMed:11491531, ECO:0000269|PubMed:11777960, ECO:0000269|PubMed:9497295}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000250|UniProtKB:Q29983}; Single-pass type I membrane protein {ECO:0000250|UniProtKB:Q29983}. Note=Binding to human cytomegalovirus glycoprotein UL16 causes sequestration in the endoplasmic reticulum. {ECO:0000250|UniProtKB:Q29983, ECO:0000269|PubMed:12782710}.
Tissues
TISSUE SPECIFICITY: Widely expressed with the exception of the central nervous system where it is absent. Expressed in many, but not all, epithelial tumors of lung, breast, kidney, ovary, prostate and colon. In hepatocellular carcinomas, expressed in tumor cells but not in surrounding non-cancerous tissue. {ECO:0000269|PubMed:10359807, ECO:0000269|PubMed:12569559, ECO:0000269|PubMed:17565371}.
Structure
SUBUNIT: Unlike classical MHC class I molecules, does not form a heterodimer with beta-2-microglobulin. Binds as a monomer to a KLRK1/NKG2D homodimer. KLRK1 forms a complex with HCST/DAP10 in which KLRK1 binds MICB while HCST acts as an adapter molecule which enables signal transduction. Receptor-ligand interaction induces clustering of both proteins in ordered structures called immune synapses and also leads to their intercellular transfer. This is associated with a reduction in the cytotoxicity of KLRK1-expressing cells. {ECO:0000250|UniProtKB:Q29983, ECO:0000269|PubMed:11239445, ECO:0000269|PubMed:11491531, ECO:0000269|PubMed:12782710, ECO:0000269|PubMed:16849432}.; SUBUNIT: (Microbial infection) Interacts with human cytomegalovirus/HHV-5 glycoprotein UL16; this interaction causes sequestration of MICB in the endoplasmic reticulum and increases resistance to KLRK1-mediated cytotoxicity. {ECO:0000269|PubMed:11239445}.
Post-translational modification
PTM: Proteolytically cleaved and released from the cell surface of tumor cells. {ECO:0000269|PubMed:16698441}.
Target Relevance information above includes information from UniProt accession : Q29980
The UniProt Consortium

Data

SPR with Biotinylated Human MICB Protein
Human NKG2D, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human MICB, His Tag with an affinity constant of 60.38 nM as determined in SPR assay (Biacore T200).
ELISA with Biotinylated Human MICB Protein
Immobilized Anti-MICB Antibody, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human MICB, His Tag with the EC50 of 0.43µg/ml determined by ELISA (QC Test).
HPLC of Biotinylated Human MICB Protein
The purity of Biotinylated Human MICB is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human MICB Protein
Biotinylated Human MICB on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




pmidtitleauthorscitation

Protocols

relevant to this product

Documents

#
No results found

Reviews

There are no reviews yet.

Only logged in customers who have purchased this product may leave a review.