ELISA with Biotinylated Human MICA Protein 4869

Biotinylated Human MICA Protein 4869

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Glu24-Gln308

SKU: 4869parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	TMICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig‑like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.
molecular weight	The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 60-68 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human MICA Protein 4869

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
TMICA (MHC class I chain-related gene A) is a transmembrane glycoprotein that functions as a ligand for human NKG2D. A closely related protein, MICB, shares 85% amino acid identity with MICA. These proteins are distantly related to the MHC class I proteins. They possess three extracellular Ig-like domains, but they have no capacity to bind peptide or interact with beta 2-microglobulin.
Protein names MHC class I chain-related protein A (MHC class I polypeptide-related sequence A) (MICA) (Stress inducible class I homolog)
Gene names MICA,MICA hCG_2001511
Mass 9606Da
Catalytic activity BINDING 248; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /evidence="ECO:0007829\|PDB:6DDR"; BINDING 249; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /evidence="ECO:0007829\|PDB:6DDR"
Target Relevance information above includes information from UniProt accession: Q96QC4
The UniProt Consortium

Data

ELISA with Biotinylated Human MICA Protein

Immobilized Anti-MICA Antibody, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 1.7µg/ml determined by ELISA (QC Test).

Immobilized Human NKG2D, hFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human MICA, His Tag with the EC50 of 0.32µg/ml determined by ELISA.

The purity of Biotinylated Human MICA is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human MICA Protein

Biotinylated Human MICA on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.