Biotinylated Human Mature TGF beta 2 Protein 3230

$600.00 – $2,000.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ala303-Ser414

SKU: 3230parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-Avi
purity	> 95% as determined by Tris-Bis PAGE
background	Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.
molecular weight	The protein has a predicted MW of 14.53 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human Mature TGF beta 2 Protein 3230

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Transforming growth factor beta(2) (TGF-beta(2)), a growth regulator of human lens epithelial cells (HLECs), also regulates the death of these cells. TGF-beta(2)-induced apoptosis in HLECs was preceded by an induction of reactive oxygen species (ROS) and a decrease in glutathione in the intracellular content, indicating that this factor induces oxidative stress in HLECs.
Protein names Transforming growth factor beta-2 proprotein (Cetermin) (Glioblastoma-derived T-cell suppressor factor) (G-TSF) [Cleaved into: Latency-associated peptide (LAP); Transforming growth factor beta-2 (TGF-beta-2)]
Gene names TGFB2,TGFB2
Protein family TGF-beta family
Mass 47748Da
Function [Transforming growth factor beta-2 proprotein]: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.; [Latency-associated peptide]: Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix (By similarity). Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 (By similarity).; [Transforming growth factor beta-2]: Multifunctional protein that regulates various processes such as angiogenesis and heart development (PubMed:22772368, PubMed:22772371). Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix (By similarity). At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus (By similarity). Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal (By similarity).
Subellular location [Latency-associated peptide]: Secreted, extracellular space, extracellular matrix .; [Transforming growth factor beta-2]: Secreted .
Structure Interacts with the serine proteases, HTRA1 and HTRA3 (By similarity). Interacts with ASPN (PubMed:17827158). Interacts with MFAP5 (By similarity).; [Latency-associated peptide]: Interacts with Transforming growth factor beta-2 (TGF-beta-2) chain; interaction is non-covalent and maintains (TGF-beta-2) in a latent state (By similarity). Interacts with LRRC32/GARP; leading to regulate activation of TGF-beta-2 (PubMed:19651619). Interacts with NREP; the interaction results in a decrease in TGFB2 autoinduction (By similarity).; [Transforming growth factor beta-2]: Homodimer; disulfide-linked (PubMed:1631557, PubMed:1641027). Interacts with TGF-beta receptors (TGFBR1 and TGFBR2), leading to signal transduction (By similarity).
Post-translational modification [Transforming growth factor beta-2 proprotein]: The precursor proprotein is cleaved in the Golgi apparatus to form Transforming growth factor beta-2 (TGF-beta-2) and Latency-associated peptide (LAP) chains, which remain non-covalently linked, rendering TGF-beta-2 inactive.
Target Relevance information above includes information from UniProt accession: P61812
The UniProt Consortium

ELISA with Biotinylated Human Mature TGF beta 2 Protein

Immobilized Biotinylated Human Mature TGF beta 2, Avi Tag at 2µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Human TGF-beta RII, mFc Tag with the EC50 of 0.27µg/ml determined by ELISA.

SDS-PAGE gel of Biotinylated Human Mature TGF beta 2 Protein

Biotinylated Human Mature TGF beta 2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

1. What is included in the package of the Biotinylated Human Mature TGF-beta 2 Protein (3230)?
- The package includes 100μg of biotinylated human mature TGF-beta 2 protein formulated in PBS.

2. What are the storage recommendations for this product?
- It is recommended to store this protein at -20°C upon receipt. Avoid repeated freeze/thaw cycles to maintain its stability and functionality.

3. Is this protein compatible with specific applications or assays?
- Yes, this biotinylated TGF-beta 2 protein is suitable for applications such as ELISA, Western blot, and immunohistochemistry among others. It can be used in various immunoassays for research purposes.

4. How can I use this biotinylated protein in my experiments?
- This protein can be used as a positive control in experiments involving TGF-beta 2 detection or for assay development. It can also be used for studying TGF-beta 2 signaling pathways in vitro.

5. Can this product be used in cell culture or in vivo experiments?
- While this protein is primarily intended for in vitro experiments, it can be used in cell culture studies and certain in vivo applications after appropriate validation and optimization.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.