ELISA with Biotinylated Human LRRC15/LIB Protein 4296

Biotinylated Human LRRC15/LIB Protein 4296

$525.00 – $1,750.00

Summary

Expression: CHO
Functional: Yes (ELISA)
Amino Acid Range: Tyr22-Gly538

SKU: 4296parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	CHO
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation. LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.
molecular weight	The protein has a predicted MW of 60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human LRRC15/LIB Protein 4296

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.Â LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.
Protein names Leucine-rich repeat-containing protein 15 (Leucine-rich repeat protein induced by beta-amyloid homolog) (hLib)
Gene names LRRC15,LRRC15 LIB
Mass 9606Da
Subellular location Cell membrane ; Single-pass type I membrane protein .
Tissues Expressed in brain and placenta (PubMed:11785964). Expressed in lung fibroblasts (PubMed:36228039, PubMed:36757924). Expressed in chodrocytes (PubMed:34702854).
Target Relevance information above includes information from UniProt accession: Q8TF66
The UniProt Consortium

Data

ELISA with Biotinylated Human LRRC15/LIB Protein

Immobilized Anti-LRRC15 Antibody, hFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human LRRC15, His Tag with the EC50 of 68.3ng/ml determined by ELISA.

HPLC of Biotinylated Human LRRC15/LIB Protein

The purity of Biotinylated Human LRRC15 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human LRRC15/LIB Protein

Biotinylated Human LRRC15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.