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Biotinylated Human LILRB2/CD85d/ILT4 Protein 4781

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gln22-His458
Weight1 lbs
Dimensions9 × 5 × 2 in
product tag

C-His-Avi, also biotinylated

Biotinylated Human LILRB2/CD85d/ILT4 Protein 4781

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.
Protein names
Leukocyte immunoglobulin-like receptor subfamily B member 2 (LIR-2) (Leukocyte immunoglobulin-like receptor 2) (CD85 antigen-like family member D) (Immunoglobulin-like transcript 4) (ILT-4) (Monocyte/macrophage immunoglobulin-like receptor 10) (MIR-10) (CD antigen CD85d)
Gene names
LILRB2,LILRB2 ILT4 LIR2 MIR10
Mass
9606Da
Function
FUNCTION: Receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles (PubMed:11169396, PubMed:12853576, PubMed:16455647, PubMed:20448110, PubMed:27859042). Involved in the down-regulation of the immune response and the development of tolerance. Recognizes HLA-G in complex with B2M/beta-2 microglobulin and a nonamer self-peptide (peptide-bound HLA-G-B2M) triggering differentiation of type 1 regulatory T cells and myeloid-derived suppressor cells, both of which actively maintain maternal-fetal tolerance (PubMed:16455647, PubMed:20448110, PubMed:27859042). Competes with CD8A for binding to class I MHC antigens. Inhibits FCGR1A-mediated phosphorylation of cellular proteins and mobilization of intracellular calcium ions (PubMed:11875462, PubMed:12853576, PubMed:9548455, PubMed:9842885). {ECO:0000269|PubMed:11169396, ECO:0000269|PubMed:11875462, ECO:0000269|PubMed:12853576, ECO:0000269|PubMed:16455647, ECO:0000269|PubMed:20448110, ECO:0000269|PubMed:27859042, ECO:0000269|PubMed:9548455, ECO:0000269|PubMed:9842885}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:20448110}; Single-pass type I membrane protein.
Tissues
TISSUE SPECIFICITY: Expressed in monocytes and at lower levels in myeloid and plasmacytoid dendritic cells. Expressed in tolerogenic IL10-producing dendritic cells (PubMed:20448110). Expressed in myeloid-derived suppressor cells during pregnancy (PubMed:27859042). Detected at low levels in natural killer (NK) cells. Expressed in B cells. {ECO:0000269|PubMed:20448110, ECO:0000269|PubMed:27859042, ECO:0000269|PubMed:9548455, ECO:0000269|PubMed:9842885}.
Structure
SUBUNIT: Binds PTPN6 when phosphorylated. Binds FCGR1A. Interacts with peptide-bound HLA-G-B2M; this interaction is direct (PubMed:16455647). Interacts with peptide-bound HLA-F-B2M; this interaction is direct (PubMed:11169396). {ECO:0000269|PubMed:11169396, ECO:0000269|PubMed:16455647}.
Post-translational modification
PTM: Phosphorylated on tyrosine residues. Dephosphorylated by PTPN6. {ECO:0000269|PubMed:9842885}.
Domain
DOMAIN: Co
Target Relevance information above includes information from UniProt accession : Q8N423
The UniProt Consortium

Data

ELISA with Biotinylated Human LILRB2/CD85d/ILT4 Protein
Immobilized Biotinylated Human LILRB2, His Tag at 0.5µg/ml (100µl/Well) on streptavidin (5µg/ml) precoated plate. Dose response curve for Anti-LILRB2 Antibody, hFc Tag with the EC50 of 4.0ng/ml determined by ELISA.
HPLC of Biotinylated Human LILRB2/CD85d/ILT4 Protein
The purity of Biotinylated Human LILRB2 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human LILRB2/CD85d/ILT4 Protein
Biotinylated Human LILRB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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