ELISA with Biotinylated Human IL-5 R alpha/CD125 Protein 3417

Biotinylated Human IL-5 R alpha/CD125 Protein 3417

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Asp21-Glu335

SKU: 3417parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Interleukin‑5 Receptor alpha (IL‑5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.
molecular weight	The protein has a predicted MW of 38.7 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per ug by the LAL method.

Biotinylated Human IL-5 R alpha/CD125 Protein 3417

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Interleukin-5 Receptor alpha (IL-5 R alpha), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology.This is the receptor for interleukin-5. The alpha chain binds to IL5.
Protein names Interleukin-5 receptor subunit alpha (IL-5 receptor subunit alpha) (IL-5R subunit alpha) (IL-5R-alpha) (IL-5RA) (CDw125) (CD antigen CD125)
Gene names IL5RA,IL5RA IL5R
Protein family Type I cytokine receptor family, Type 5 subfamily
Mass 9606Da
Function Cell surface receptor that plays an important role in the survival, differentiation, and chemotaxis of eosinophils (PubMed:9378992). Acts by forming an heterodimeric receptor with CSF2RB subunit and subsequently binding to interleukin-5 (PubMed:1495999, PubMed:22528658). In unstimulated conditions, interacts constitutively with JAK2. Heterodimeric receptor activation leads to JAK2 stimulation and subsequent activation of the JAK-STAT pathway (PubMed:9516124).
Subellular location Membrane; Single-pass type I membrane protein.
Tissues Expressed on eosinophils and basophils.
Structure Interacts with IL5 (PubMed:1495999, PubMed:22153509, PubMed:22528658). Interacts with CSF2RB (PubMed:9516124). Interacts with JAK2 (PubMed:9516124). Interacts with SDCBP (PubMed:12842047).
Domain Th
Target Relevance information above includes information from UniProt accession: Q01344
The UniProt Consortium

Data

ELISA with Biotinylated Human IL-5 R alpha/CD125 Protein

Immobilized Human IL-5, His Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human IL-5 R alpha, His Tag with the EC50 of 0.15µg/ml determined by ELISA.

HPLC of Biotinylated Human IL-5 R alpha/CD125 Protein

The purity of Biotinylated Human IL-5 R alpha is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human IL-5 R alpha/CD125 Protein

Biotinylated Human IL-5 R alpha on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.