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Biotinylated Human IL-10 Protein 4881

$600.00$2,000.00

Summary

  • Expression: HEK293
  • Binding assay: Yes (SPR)
  • Amino Acid Range: Ser19-Asn178
SKU: 4881parent Categories: , Tags: , , ,
Weight1 lbs
Dimensions9 × 5 × 2 in
product tag

N-His-Avi, also biotinylated

Biotinylated Human IL-10 Protein 4881

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Interleukin 10 (IL10) is a key anti-inflammatory cytokine that can inhibit proinflammatory responses of both innate and adaptive immune cells. An association between IL10 and intestinal mucosal homeostasis became clear with the discovery that IL10 and IL10 receptor (IL10R)-deficient mice develop spontaneous intestinal inflammation. Similarly, patients with deleterious mutations in IL10, IL10RA, or IL10RB present with severe enterocolitis within the first months of life.
Protein names
Interleukin-10 (IL-10) (Cytokine synthesis inhibitory factor) (CSIF)
Gene names
IL10,IL10
Protein family
IL-10 family
Mass
9606Da
Function
FUNCTION: Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3 (PubMed:16982608). In turn, STAT3 translocates to the nucleus where it drives expression of anti-inflammatory mediators (PubMed:18025162). Targets antigen-presenting cells (APCs) such as macrophages and monocytes and inhibits their release of pro-inflammatory cytokines including granulocyte-macrophage colony-stimulating factor /GM-CSF, granulocyte colony-stimulating factor/G-CSF, IL-1 alpha, IL-1 beta, IL-6, IL-8 and TNF-alpha (PubMed:11564774, PubMed:1940799, PubMed:7512027). Interferes also with antigen presentation by reducing the expression of MHC-class II and co-stimulatory molecules, thereby inhibiting their ability to induce T cell activation (PubMed:8144879). In addition, controls the inflammatory response of macrophages by reprogramming essential metabolic pathways including mTOR signaling (By similarity). {ECO:0000250|UniProtKB:P18893, ECO:0000269|PubMed:11564774, ECO:0000269|PubMed:16982608, ECO:0000269|PubMed:18025162, ECO:0000269|PubMed:1940799, ECO:0000269|PubMed:7512027, ECO:0000269|PubMed:8144879}.
Subellular location
SUBCELLULAR LOCATION: Secreted.
Tissues
TISSUE SPECIFICITY: Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. {ECO:0000269|PubMed:1940799, ECO:0000269|PubMed:7512027}.
Structure
SUBUNIT: Homodimer (PubMed:11485736, PubMed:15837194). Interacts with IL10RA and IL10RB (PubMed:16982608). {ECO:0000269|PubMed:11485736, ECO:0000269|PubMed:15837194, ECO:0000269|PubMed:16982608}.
Target Relevance information above includes information from UniProt accession : P22301
The UniProt Consortium

Data

SPR with Biotinylated Human IL-10 Protein
Human IL-10 R alpha, His Tag immobilized on CM5 Chip can bind Biotinylated Human IL-10, His Tag with an affinity constant of 0.60 nM as determined in SPR assay (Biacore T200).
SDS-PAGE gel of Biotinylated Human IL-10 Protein
Biotinylated Human IL-10 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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