ELISA with Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Mon 4568

HPLC of Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monome 4568

SDS-PAGE gel of Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC 4568

Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein 4568

$310.00 – $1,250.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and HMTEVVRHC peptide

SKU: 4568parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.
molecular weight	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 55-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein 4568

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.
Protein names Major histocompatibility complex, class I, A
Gene names HLA-A,HLA-A
Protein family MHC class I family
Mass 41449Da
Target Relevance information above includes information from UniProt accession: A0A140T913
The UniProt Consortium

Data

ELISA with Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Mon

Immobilized Anti-HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Antibody, hFc Tag at 5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer, His Tag with the EC50 of 0.25µg/ml determined by ELISA.

HPLC of Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monome

The purity of Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer was greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC

Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.