ELISA with Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Mono 4351

HPLC of Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer 4351

SDS-PAGE gel of Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) 4351

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein 4351

$310.00 – $1,250.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and GVYDGREHTV peptide

SKU: 4351parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A0201 MAGE-A4 (GVYDGREHTV) Complex Protein is a complex of HLA-A0201 of the MHC Class I, B2M and GVYDGREHTV peptide of the MAGE-A4.
molecular weight	The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer Protein 4351

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Melanoma-associated antigen 4 is a protein that in humans is encoded by the MAGEA4 gene.The MAGE- A4 antigen is among the most commonly expressed cancer testis antigens. The Human HLA-A0201 MAGE-A4 (GVYDGREHTV) Complex Protein is a complex of HLA-A0201 of Â the MHC Class I, B2M and GVYDGREHTV peptide of Â the MAGE-A4.
Protein names Major histocompatibility complex, class I, A
Gene names HLA-A,HLA-A
Protein family MHC class I family
Mass 41449Da
Target Relevance information above includes information from UniProt accession: A0A140T913
The UniProt Consortium

Data

ELISA with Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Mono

Immobilized Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer, His Tag at 0.5µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Antibpdy, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA.

HPLC of Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer

The purity of Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer was greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV)

Biotinylated Human HLA-A*02:01&B2M&MAGE-A4 (GVYDGREHTV) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.