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Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein 4626

$310.00$1,250.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YLEPGPVTA peptide
SKU: 4626parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of  the MHC Class I and YLEPGPVTA peptide of  the gp100.

molecular weight

The protein has a predicted MW of 50.4 kDa. Due to glycosylation, the protein migrates to 52-62 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Protein 4626

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of  the MHC Class I and YLEPGPVTA peptide of  the gp100.
Protein names
Major histocompatibility complex, class I, A
Gene names
HLA-A,HLA-A
Protein family
MHC class I family
Mass
41449Da
Target Relevance information above includes information from UniProt accession: A0A140T913
The UniProt Consortium

Data

ELISA with Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer
Immobilized Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag at 2µg/ml (100µl/Well) on the streptavidin precoated plate (5µg/ml). Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.7ng/ml determined by ELISA (QC Test).
ELISA with Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer
Immobilized Tebentafusp, His Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer, His Tag with the EC50 of 11.8ng/ml determined by ELISA.
HPLC of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer Pr
The purity of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer was greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Mo
Biotinylated Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Monomer on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

relevant to this product

Documents

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