ELISA with Biotinylated Human Her3/ErbB3 Protein 4846

Biotinylated Human Her3/ErbB3 Protein 4846

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ser20-Thr643

SKU: 4846parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
background	Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).ation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
molecular weight	The protein has a predicted MW of 71.6 kDa. Due to glycosylation, the protein migrates to 90-100 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1 EU per μg by the LAL method.

Biotinylated Human Her3/ErbB3 Protein 4846

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Her3, also called ErbB3, is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors.Her3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric Her3 serves as a low affinity receptor for the heregulins (HRG).ation leads to a deletion of exons 2-7 of the EGFR gene and renders the mutant receptor incapable of binding any known ligand.
Protein names Receptor tyrosine-protein kinase erbB-3 (EC 2.7.10.1) (Proto-oncogene-like protein c-ErbB-3) (Tyrosine kinase-type cell surface receptor HER3)
Protein family Protein kinase superfamily, Tyr protein kinase family, EGF receptor subfamily
Mass 148098Da
Function Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins. Binds to neuregulin-1 (NRG1) and is activated by it; ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). May also be activated by CSPG5 (PubMed:15358134). Involved in the regulation of myeloid cell differentiation (PubMed:27416908). {ECO:0000269\|PubMed:15358134, ECO:0000269\|PubMed:20682778, ECO:0000269\|PubMed:27416908}.
Catalytic activity CATALYTIC ACTIVITY: Reaction=ATP + L-tyrosyl-[protein] = ADP + H(+) + O-phospho-L-tyrosyl-[protein]; Xref=Rhea:RHEA:10596, Rhea:RHEA-COMP:10136, Rhea:RHEA-COMP:10137, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:46858, ChEBI:CHEBI:82620, ChEBI:CHEBI:456216; EC=2.7.10.1; Evidence={ECO:0000269\|PubMed:20351256};
Subellular location [Isoform 1]: Cell membrane {ECO:0000269\|PubMed:33497358}; Single-pass type I membrane protein.; [Isoform 2]: Secreted.
Tissues Epithelial tissues and brain.
Structure Monomer and homodimer. Heterodimer with each of the other ERBB receptors (Potential). Interacts with CSPG5 (PubMed:15358134). Interacts with GRB7 (PubMed:9516479). Interacts with MUC1 (PubMed:12939402). Interacts with MYOC (By similarity). Interacts with isoform 2 of PA2G4 (PubMed:11325528, PubMed:16832058). Found in a ternary complex with NRG1 and ITGAV:ITGB3 or ITGA6:ITGB4 (PubMed:20682778). {ECO:0000250\|UniProtKB:Q61526, ECO:0000269\|PubMed:11325528, ECO:0000269\|PubMed:12939402, ECO:0000269\|PubMed:15358134, ECO:0000269\|PubMed:16832058, ECO:0000269\|PubMed:20682778, ECO:0000269\|PubMed:9516479, ECO:0000305}.
Post-translational modification Autophosphorylated (PubMed:20351256). Ligand-binding increases phosphorylation on tyrosine residues and promotes its association with the p85 subunit of phosphatidylinositol 3-kinase (PubMed:20682778). {ECO:0000269\|PubMed:20351256, ECO:0000269\|PubMed:20682778, ECO:0000269\|PubMed:33497358}.
Domain The cytoplasmic part of the receptor may interact with the SH2 or SH3 domains of many signal
Target Relevance information above includes information from UniProt accession: P21860
The UniProt Consortium

Data

ELISA with Biotinylated Human Her3/ErbB3 Protein

Immobilized Biotinylated Human Her3, His Tag at 2 µg/ml (100 µl/Well) on the streptavidin precoated plate (5 µg/ml). Dose response curve for Human NRG1 Beta 1, hFc Tag with the EC50 of 33.3 ng/ml determined by ELISA (QC Test).

Immobilized Anti-Her3 Antibody, hFc Tag at 2 µg/ml (100 µl/Well) on the plate. Dose response curve for Biotinylatd Human Her3, His Tag with the EC50 of 58.1 ng/ml determined by ELISA.

HPLC of Biotinylated Human Her3/ErbB3 Protein

The purity of Biotinylated Human Her3 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human Her3/ErbB3 Protein

Biotinylated Human Her3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.