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Biotinylated Human GPRC5D Protein-VLP 4303

$1,969.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Met1-Val345
SKU: 4303parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
accession

Q9NZD1

express system

HEK293

product tag
purity

> 95% as determined by HPLC

background

Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.

molecular weight

The target protein has a predicted MW of 39.6 kDa.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μl by the LAL method.

Biotinylated Human GPRC5D Protein-VLP 4303

protein
Size and concentration
100, 500µg and liquid
Form
Liquid
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped with dry ice.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Chimeric antigen receptor (CAR) T cells, a type of cell-based immunotherapy, have shown some promising results in multiple myeloma, a bone marrow cancer.The orphan G protein–coupled receptor, class C group 5 member D (GPRC5D), normally expressed only in the hair follicle, Using quantitative immunofluorescence, we determined that GPRC5D protein is expressed on CD138 MM cells from primary marrow samples with a distribution that was similar to, but independent of, BCMA.
Protein names
G-protein coupled receptor family C group 5 member D
Gene names
GPRC5D,GPRC5D
Protein family
G-protein coupled receptor 3 family
Mass
9606Da
Subellular location
Cell membrane ; Multi-pass membrane protein .
Tissues
Widely expressed in the peripheral system. Expression pattern is high in pancreas, medium in kidney, small intestine, spleen and testis, low in lung, colon, leukocyte, prostate and thymus and not detectable in brain, heart, liver, placenta, skeletal muscle and ovary.
Target Relevance information above includes information from UniProt accession: Q9NZD1
The UniProt Consortium

Data

SPR with Biotinylated Human GPRC5D Protein-VLP
Biotinylated Human GPRC5D VLP captured on SA Chip can bind Anti-GPRC5D antibody, hFc Tag with an affinity constant of 0.30 nM as determined in SPR assay (Biacore T200) (QC Test).
ELISA with Biotinylated Human GPRC5D Protein-VLP
Immobilized Human CD3E&CD3D, hFc Tag at 1µg/ml (100µl/Well) on the plate. Add serial dilutions of Anti-CD3 GPRC5D Bispecific Antibody, hFc Tag in 10% Human Serum and then add various dilution ratio of Biotinylated Human GPRC5D VLP. Detection was performed using HRP-conjugated streptavidin with the EC50 of 75.5µg/ml determined by ELISA.
HPLC of Biotinylated Human GPRC5D Protein-VLP
The purity of Biotinylated Human GPRC5D VLP is greater than 95% as determined by SEC-HPLC.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

relevant to this product

Documents

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