ELISA with Biotinylated Human GPA33/A33 Protein 4769

Biotinylated Human GPA33/A33 Protein 4769

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (ELISA)
Amino Acid Range: Ile22-Val235

SKU: 4769parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
molecular weight	The protein has a predicted MW of 26.5 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human GPA33/A33 Protein 4769

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Glycoprotein A33 (GPA33) is also known as Cell surface A33 antigen, is a single-pass type I membrane protein which is expressed in normal gastrointestinal epithelium and in 95% of colon cancers. GPA33 The predicted mature protein has a 213-amino acid extracellular region, a single transmembrane domain, and a 62-amino acid intracellular tail. The sequence of the extracellular region contains 1 Ig-like C2-type (immunoglobulin-like) domain and 1 Ig-like V-type (immunoglobulin-like) domain characteristic of the CD2 subgroup of the immunoglobulin (Ig) superfamily, which contains. GPA33 may play a role in cell-cell recognition and signaling.
Protein names Cell surface A33 antigen (Glycoprotein A33)
Mass 35632Da
Function May play a role in cell-cell recognition and signaling.
Subellular location Membrane; Single-pass type I membrane protein.
Tissues Expressed in normal gastrointestinal epithelium and in 95% of colon cancers.
Post-translational modification N-glycosylated, contains approximately 8 kDa of N-linked carbohydrate. {ECO:0000269\|PubMed:9245713}.; Palmitoylated. {ECO:0000269\|PubMed:9245713}.
Target Relevance information above includes information from UniProt accession: Q99795
The UniProt Consortium

Data

ELISA with Biotinylated Human GPA33/A33 Protein

Immobilized Anti-GPA33 Antibody, hFc Tag at 0.5µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human GPA33, His Tag with the EC50 of 33.2ng/ml determined by ELISA.

HPLC of Biotinylated Human GPA33/A33 Protein

The purity of Biotinylated Human GPA33 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human GPA33/A33 Protein

Biotinylated Human GPA33 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.