ELISA with Biotinylated Human GFRAL/GFR alpha-like Protein 4135

Binding assay with Biotinylated Human GFRAL/GFR alpha-like Protein 4135

Biotinylated Human GFRAL/GFR alpha-like Protein 4135

$525.00 – $1,750.00

Summary

Expression: HEK293
Functional: Yes (binding assay)
Amino Acid Range: Ser19-Glu351

SKU: 4135parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
molecular weight	The protein has a predicted MW of 40.7 kDa. Due to glycosylation, the protein migrates to 48-62 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human GFRAL/GFR alpha-like Protein 4135

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
GFR alpha -like (GDNF receptor-alpha-like) is a distant member of the GDNFR family of proteins. Mature human GFR alpha-like is a 376 amino acid (aa) type I transmembrane protein. It contains a 333 aa extracellular domain, a 20 aa transmembrane domain and a 23 aa cytoplasmic domain.GFRAL is a brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses.
Protein names GDNF family receptor alpha-like
Gene names GFRAL,GFRAL C6orf144 UNQ9356/PRO34128
Protein family GDNFR family
Mass 9606Da
Function Brainstem-restricted receptor for GDF15 hormone, which triggers an aversive response, characterized by nausea, vomitting, and/or loss of appetite in response to various stresses (PubMed:28846097, PubMed:28846098, PubMed:28846099, PubMed:28953886, PubMed:36630958). The aversive response is both required to reduce continuing exposure to those stresses at the time of exposure and to promote avoidance behavior in the future (PubMed:28846097, PubMed:28846098, PubMed:28846099, PubMed:28953886, PubMed:36630958). The GDF15-GFRAL aversive response is triggered by stresses, such as anticancer drugs (camptothecin or cisplatin), cancers or drugs such as metformin (PubMed:32661391). Upon interaction with its ligand, GDF15, mediates the GDF15-induced autophosphorylation and activation of the RET tyrosine kinase receptor, leading to activation of MAPK- and AKT- signaling pathways (PubMed:31535977, PubMed:32661391). Ligand-binding activates GFRAL-expressing neurons localized in the area postrema and nucleus tractus solitarius of the brainstem (By similarity). The GDF15-GFRAL signal induces expression of genes involved in metabolism, such as lipid metabolism in adipose tissues (PubMed:32661391).
Subellular location Cell membrane ; Single-pass membrane protein ; Extracellular side .
Tissues Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius (at protein level) (PubMed:28846097, PubMed:28846098). Detected at low levels in testis and adipose tissue (PubMed:28846097).
Structure Interacts (via the extracellular domain) with GDF15 and RET; receptor of GDF15, mediates cellular signaling through interaction with RET after GDF15-binding (PubMed:28846097, PubMed:28846099, PubMed:28953886, PubMed:31535977). Interaction with RET requires previous GDF15-binding (PubMed:28846097, PubMed:28846099).
Post-translational modification Cleaved and inactivated by MMP14, inhibiting the GDF15-GFRAL aversive response.
Target Relevance information above includes information from UniProt accession: Q6UXV0
The UniProt Consortium

Data

ELISA with Biotinylated Human GFRAL/GFR alpha-like Protein

Immobilized Human GDF15, His Tag at 1µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human GFRAL, His Tag with the EC50 of 89.1ng/ml determined by ELISA (QC Test).

Binding assay with Biotinylated Human GFRAL/GFR alpha-like Protein

Serial dilutions of Anti-GDF15 Antibody were added into Human GDF15, His Tag : Biotinylated Human GFRAL, His Tag binding reactioins. The half maximal inhibitiory concentration (IC50) is 40.8ng/ml.

HPLC of Biotinylated Human GFRAL/GFR alpha like Protein

The purity of Biotinylated Human GFRAL is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human GFRAL/GFR alpha-like Protein

Biotinylated Human GFRAL on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.