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Biotinylated Human FGFR2 alpha (IIIb) Protein 4783



  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Arg22-Glu378
Weight1 lbs
Dimensions9 × 5 × 2 in
product tag

C-His-Avi, also biotinylated

Biotinylated Human FGFR2 alpha (IIIb) Protein 4783

Size and concentration
100, 500µg and lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
> 95% as determined by Tris-Bis PAGE
target relevance
Four distinct genes encoding closely related FGF receptors, FGF R1 - 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII domains, a transmembrane domain and the split tyrosine-kinase domain. Multiple forms of FGF R1 - 3 are generated by alternative splicing of the mRNAs.
Protein names
Fibroblast growth factor receptor 2 (FGFR-2) (EC (K-sam) (KGFR) (Keratinocyte growth factor receptor) (CD antigen CD332)
Gene names
Protein family
Protein kinase superfamily, Tyr protein kinase family, Fibroblast growth factor rec
FUNCTION: Tyrosine-protein kinase that acts as a cell-surface receptor for fibroblast growth factors and plays an essential role in the regulation of cell proliferation, differentiation, migration and apoptosis, and in the regulation of embryonic development. Required for normal embryonic patterning, trophoblast function, limb bud development, lung morphogenesis, osteogenesis and skin development. Plays an essential role in the regulation of osteoblast differentiation, proliferation and apoptosis, and is required for normal skeleton development. Promotes cell proliferation in keratinocytes and immature osteoblasts, but promotes apoptosis in differentiated osteoblasts. Phosphorylates PLCG1, FRS2 and PAK4. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. FGFR2 signaling is down-regulated by ubiquitination, internalization and degradation. Mutations that lead to constitutive kinase activation or impair normal FGFR2 maturation, internalization and degradation lead to aberrant signaling. Over-expressed FGFR2 promotes activation of STAT1. {ECO:0000269|PubMed:12529371, ECO:0000269|PubMed:15190072, ECO:0000269|PubMed:15629145, ECO:0000269|PubMed:16384934, ECO:0000269|PubMed:16597617, ECO:0000269|PubMed:17311277, ECO:0000269|PubMed:17623664, ECO:0000269|PubMed:18374639, ECO:0000269|PubMed:19103595, ECO:0000269|PubMed:19387476, ECO:0000269|PubMed:19410646, ECO:0000269|PubMed:21596750, ECO:0000269|PubMed:8663044}.
Subellular location
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. Golgi apparatus. Cytoplasmic vesicle. Note=Detected on osteoblast plasma membrane lipid rafts. After ligand binding, the activated receptor is rapidly internalized and degraded.; SUBCELLULAR LOCATION: [Isoform 1]: Cell membrane; Single-pass type I membrane protein. Note=After ligand binding, the activated receptor is rapidly internalized and degraded.; SUBCELLULAR LOCATION: [Isoform 3]: Cell membrane; Single-pass type I membrane protein. Note=After ligand binding, the activated receptor is rapidly internalized and degraded.; SUBCELLULAR LOCATION: [Isoform 8]: Secreted.; SUBCELLULAR LOCATION: [Isoform 13]: Secreted.
SUBUNIT: Monomer. Homodimer after ligand binding. Interacts predominantly with FGF1 and FGF2, but can also interact with FGF3, FGF4, FGF6, FGF7, FGF8, FGF9, FGF10, FGF17, FGF18 and FGF22 (in vitro). Ligand specificity is determined by tissue-specific expression of isoforms, and differences in the third Ig-like domain are crucial for ligand specificity. Isoform 1 has high affinity for FGF1 and FGF2, but low affinity for FGF7. Isoform 3 has high affinity for FGF1 and FGF7, and has much higher affinity for FGF7 than isoform 1 (in vitro). Affinity for fibroblast growth factors (FGFs) is increased by heparan sulfate glycosaminoglycans that function as coreceptors. Likewise, KLB increases the affinity for FGF19 and FGF21. Interacts with PLCG1, GRB2 and PAK4. Interacts with FLRT2 (By similarity). {ECO:0000250|UniProtKB:P21803, ECO:0000269|PubMed:10618369, ECO:0000269|PubMed:10830168, ECO:0000269|PubMed:11069186, ECO:0000269|PubMed:11390973, ECO:0000269|PubMed:12529371, ECO:0000269|PubMed:12591959, ECO:0000269|PubMed:1309608, ECO:0000269|PubMed:1400433, ECO:0000269|PubMed:15190072, ECO:0000269|PubMed:15629145, ECO:0000269|PubMed:16384934, ECO:0000269|PubMed:16597617, ECO:0000269|PubMed:16844695, ECO:0000269|PubMed:17623664, ECO:0000269|PubMed:17803937, ECO:0000269|PubMed:18056630, ECO:0000269|PubMed:19060208, ECO:0000269|PubMed:19103595, ECO:0000269|PubMed:21454610, ECO:0000269|PubMed:8663044, ECO:0000269|PubMed:8961926}.
Post-translational modification
PTM: Autophosphorylated. Binding of FGF family members together with heparan sulfate proteoglycan or heparin promotes receptor dimerization and autophosphorylation on several tyrosine residues. Autophosphorylation occurs in trans between the two FGFR molecules present in the dimer. Phosphorylation at Tyr-769 is essential for interaction with PLCG1. {ECO:0000269|PubMed:15629145, ECO:0000269|PubMed:19060208}.; PTM: N-glycosylated in the endoplasmic reticulum. The N-glycan chains undergo further maturation to an Endo H-resistant form in the Golgi apparatus. {ECO:0000269|PubMed:16844695, ECO:0000269|PubMed:17311277}.; PTM: Ubiquitinated. FGFR2 is rapidly ubiquitinated after autophosphorylation, leading to internalization and degradation. Subject to degradation both in lysosomes and by the proteasome. {ECO:0000269|PubMed:15190072, ECO:0000269|PubMed:16844695, ECO:0000269|PubMed:21596750}.
Target Relevance information above includes information from UniProt accession : P21802
The UniProt Consortium


ELISA with Biotinylated Human FGFR2 alpha (IIIb) Protein
Immobilized Anti-FGFR2 alpha (IIIb) Antibody, hFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human FGFR2 alpha (IIIb) , His Tag with the EC50 of 20.3ng/ml determined by ELISA.
HPLC of Biotinylated Human FGFR2 alpha (IIIb) Protein
The purity of Biotinylated Human FGFR2 alpha (IIIb) is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human FGFR2 alpha (IIIb) Protein
Biotinylated Human FGFR2 alpha (IIIb) on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%.


Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.



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