SDS-PAGE gel of Biotinylated Human FGF-7/KGF Protein (Primary Amine Lab 3480

Biotinylated Human FGF-7/KGF Protein (Primary Amine Labeling) 3480

$600.00 – $2,000.00

Summary

Expression: E.coli
Pure: Yes (HPLC)
Amino Acid Range: Cys32-Thr194

SKU: 3480parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	E.coli
product tag	N-His
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney.
molecular weight	The protein has a predicted MW of 20.1 kDa same as Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per ug by the LAL method.

Biotinylated Human FGF-7/KGF Protein (Primary Amine Labeling) 3480

protein

Size and concentration 100, 500µg and liquid
Form Liquid
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped with dry ice.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The expression patterns of mRNAs encoding Fibroblast Growth Factor-7 (FGF-7) and its high affinity receptor suggested that FGF-7 signaling may play a role in regulating ureteric bud growth.t Results of these studies demonstrate that the developing ureteric bud and mature collecting system of FGF-7-null kidneys is markedly smaller than wild type. FGF-7 levels modulate the extent of ureteric bud growth during development and the number of nephrons that eventually form in the kidney.
Protein names Fibroblast growth factor 7 (FGF-7) (Heparin-binding growth factor 7) (HBGF-7) (Keratinocyte growth factor)
Gene names FGF7,FGF7 KGF
Protein family Heparin-binding growth factors family
Mass 9606Da
Function Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation.
Subellular location Secreted.
Tissues Epithelial cell.
Structure Interacts with FGFBP1. Interacts with FGFR2. Affinity between fibroblast growth factors (FGFs) and their receptors is increased by heparan sulfate glycosaminoglycans that function as coreceptors.
Target Relevance information above includes information from UniProt accession: P21781
The UniProt Consortium

HPLC of Biotinylated Human FGF-7/KGF Protein (Primary Amine Labeling)

The purity of Biotinylated Human FGF-7 is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human FGF-7/KGF Protein (Primary Amine Lab

Biotinylated Human FGF-7 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Frequently Asked Questions

1. What is the concentration of the biotinylated human FGF-7 (KGF) protein?
The concentration of the biotinylated human FGF-7 (KGF) protein is 0.5 mg/ml.

2. How should the biotinylated FGF-7 protein be stored?
The protein should be stored at -20°C upon arrival. For long-term storage, it is recommended to aliquot and store at -80°C to maintain its stability.

3. Is the biotinylated FGF-7 protein compatible with all common laboratory techniques?
Yes, the biotinylated FGF-7 protein is compatible with commonly used laboratory techniques such as ELISA, Western blot, and immunoprecipitation.

4. What applications is the biotinylated human FGF-7 (KGF) protein suitable for?
The biotinylated FGF-7 protein can be used for various applications including cell culture studies, in vitro assays, and as a standard in immunoassays.

5. Can the biotinylated FGF-7 protein be used for in vivo experiments?
While the protein is primarily designed for in vitro applications, it may be used for in vivo studies with proper validation and experimental controls. It is recommended to consult with a scientific expert before using the protein for in vivo experiments.

Publications

pmid	title	authors	citation
We haven't added any publications to our database yet.

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.