SPR with Biotinylated Human FcRn Protein 4138

Biotinylated Human FcRn Protein 4138

$525.00 – $1,750.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Ala24-Ser297(FCGRT) & Ile21-Met119(B2M)

SKU: 4138parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14, and forms an MHC class I-like heterodimer.
molecular weight	The protein has a predicted MW of 46.5 kDa. Due to glycosylation, the protein migrates to 48-55 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human FcRn Protein 4138

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
The neonatal Fc receptor (FCRN) is an approximately 45 kDa transmembrane glycoprotein with structural homology to MHC class I proteins. It is widely expressed in endothelial and epithelial cells and plays an important role in IgG homeostasis and antigen presentation by dendritic cells.FCGRT & B2M heterodimer protein (FcRn complex) consist of two subunits: p51, and p14 , and forms an MHC class I-like heterodimer.
Protein names IgG receptor FcRn large subunit p51 (FcRn) (IgG Fc fragment receptor transporter alpha chain) (Neonatal Fc receptor)
Protein family Immunoglobulin superfamily
Mass 39743Da
Function Cell surface receptor that transfers passive humoral immunity from the mother to the newborn. Binds to the Fc region of monomeric immunoglobulin gamma and mediates its selective uptake from milk (PubMed:10933786, PubMed:7964511). IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. Throughout life, contributes to effective humoral immunity by recycling IgG and extending its half-life in the circulation. Mechanistically, monomeric IgG binding to FcRn in acidic endosomes of endothelial and hematopoietic cells recycles IgG to the cell surface where it is released into the circulation (PubMed:10998088). In addition of IgG, regulates homeostasis of the other most abundant circulating protein albumin/ALB (PubMed:24469444, PubMed:28330995). {ECO:0000250\|UniProtKB:P13599, ECO:0000269\|PubMed:10933786, ECO:0000269\|PubMed:10998088, ECO:0000269\|PubMed:24469444, ECO:0000269\|PubMed:28330995, ECO:0000269\|PubMed:7964511}.; (Microbial infection) Acts as an uncoating receptor for a panel of echoviruses including Echovirus 5, 6, 7, 9, 11, 13, 25 and 29. {ECO:0000269\|PubMed:30808762, ECO:0000269\|PubMed:31104841}.
Subellular location Cell membrane {ECO:0000250\|UniProtKB:P13599}; Single-pass type I membrane protein {ECO:0000255}. Endosome membrane {ECO:0000269\|PubMed:31104841}.
Tissues Expressed in full-term placenta, heart, lung, liver, muscle, kidney, pancreas, and both fetal and adult small intestine. {ECO:0000269\|PubMed:28330995, ECO:0000269\|PubMed:7964511}.
Structure FcRn complex consists of two subunits: p51, and p14 which is equivalent to beta-2-microglobulin. It forms an MHC class I-like heterodimer (By similarity). Interacts with albumin/ALB; this interaction regulates ALB homeostasis (PubMed:24469444, PubMed:28330995). {ECO:0000250\|UniProtKB:P13599, ECO:0000269\|PubMed:24469444, ECO:0000269\|PubMed:28330995}.; (Microbial infection) Interacts with Echovirus 6, Echovirus 11 and Echovirus 30 capsid protein VP1. {ECO:0000269\|PubMed:30808762, ECO:0000269\|PubMed:31104841}.
Target Relevance information above includes information from UniProt accession: P55899
The UniProt Consortium

Data

SPR with Biotinylated Human FcRn Protein

Biotinylated Human FcRn, His Tag captured on CM5 Chip via anti-his antibody can bind Trastuzumab with an affinity constant of 0.557 uM asdetermined in SPR assay (Biacore T200).

The purity of Biotinylated Human FcRn is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human FcRn Protein

Biotinylated Human FcRn on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.