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Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) 2410

$600.00$2,000.00

Summary

  • Expression: HEK293
  • Functional: Yes (ELISA)
  • Amino Acid Range: Gln16-Gly851
SKU: 2410parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
express system

HEK293

product tag

C-His

purity

> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC

background

FcRH5 is a cell surface marker enriched on malignant plasma cells when compared to other hematologic malignancies and normal tissues. DFRF4539A is an anti-FcRH5 antibody-drug conjugated to monomethyl auristatin E (MMAE), a potent anti-mitotic agent.

molecular weight

The protein has a predicted MW of 92.38 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling) 2410

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
FcRH5 is a cell surface marker enriched on malignant plasma cells when compared to other hematologic malignancies and normal tissues. DFRF4539A is an anti-FcRH5 antibody-drug conjugated to monomethyl auristatin E (MMAE), a potent anti-mitotic agent.
Protein names
Fc receptor-like protein 5 (FcR-like protein 5) (FcRL5) (BXMAS1) (Fc receptor homolog 5) (FcRH5) (Immune receptor translocation-associated protein 2) (CD antigen CD307e)
Mass
106437Da
Function
Plays an important role in B-cell response to antigen that acts both as a negative or positive coreceptor. Inhibits B-cell receptor (BCR) signaling in the absence of CR2 stimulation but engagement with CR2 and the BCR lead to a superior calcium response compared to CR2 and BCR costimulation (PubMed:30107486). May be involved in B-cell development and differentiation in peripheral lymphoid organs and may be useful markers of B-cell stages. May have an immunoregulatory role in marginal zone B-cells. May play a role in fertilization (By similarity). {ECO:0000250|UniProtKB:Q68SN8, ECO:0000269|PubMed:11453668}.
Subellular location
Cell membrane {ECO:0000269|PubMed:16849395}; Single-pass type I membrane protein {ECO:0000269|PubMed:16849395}.
Tissues
Expressed in marginal zone B-cells, immunoblasts, tonsillar germinal center centrocytes and in the intraepithelial and interfollicular regions of the tonsil. Expressed in many lymphoma cell lines and on hairy cell leukemia cells. Isoform 1, isoform 3, isoform 4 and isoform 5 are detected in lymph node, spleen, bone marrow, and small intestine with preponderance of isoform 3. Expressed in mature and memory B-cells and down-regulated in germinal center cells (at protein level). {ECO:0000269|PubMed:11290337, ECO:0000269|PubMed:11929751, ECO:0000269|PubMed:16849395}.
Structure
Interacts with CR2 (PubMed:30107486). Interacts with CD19 (PubMed:30107486). {ECO:0000269|PubMed:30107486}.
Domain
Contains 2 copies of a cytoplasmic motif that is referred to as the immunoreceptor tyrosine-
Target Relevance information above includes information from UniProt accession: Q96RD9
The UniProt Consortium

ELISA with Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeli
Immobilized Anti-FcRH5 Antibody, hFc Tag at 2µg/ml (100µl/Well) on the plate. Dose response curve for Biotinylated Human FcRH5, His Tag with the EC50 of 46.0ng/ml determined by ELISA.
HPLC of Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine Labeling)
The purity of Biotinylated Human FcRH5 is greater than 90% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human FcRH5/FcRL5 Protein (Primary Amine L
Biotinylated Human FcRH5 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

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We haven't added any publications to our database yet.
Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.

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