SPR with Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein 4821

Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein 4821

$525.00 – $1,750.00

Summary

Expression: HEK293
Binding assay: Yes (SPR)
Amino Acid Range: Gly17-Ser200(NA2)

SKU: 4821parent Categories: proteins, Recombinant proteins Tag: active proteins

Weight	1 lbs
Dimensions	9 × 5 × 2 in
express system	HEK293
product tag	C-His-Avi
purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
background	Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
molecular weight	The protein has a predicted MW of 23.7 kDa. Due to glycosylation, the protein migrates to 47-53 kDa based on Tris-Bis PAGE result.
available size	100 µg, 500 µg
endotoxin	Less than 1EU per μg by the LAL method.

Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein 4821

protein

Size and concentration 100, 500µg and lyophilized
Form Lyophilized
Storage Instructions Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer Shipped at ambient temperature.
Purity > 95% as determined by Tris-Bis PAGE

target relevance
Human Fc gamma RIIIB/CD16b Protein is a receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Protein names Low affinity immunoglobulin gamma Fc region receptor III-B (Fc-gamma RIII-beta) (CD16-I) (Fc-gamma RIII) (Fc-gamma RIIIb) (FcRIII) (FcRIIIb) (FcR-10) (IgG Fc receptor III-1) (CD antigen CD16b)
Protein family 411
Function Receptor for the Fc region of immunoglobulins gamma. Low affinity receptor. Binds complexed or aggregated IgG and also monomeric IgG. Contrary to III-A, is not capable to mediate antibody-dependent cytotoxicity and phagocytosis. May serve as a trap for immune complexes in the peripheral circulation which does not activate neutrophils.
Pathway 305
Subellular location Cell membrane ; Lipid-anchor, GPI-anchor. Secreted. Note=Secreted after cleavage.
Tissues Expressed specifically by polymorphonuclear leukocytes (neutrophils). Also expressed by stimulated eosinophils.
Structure Monomer. Interacts with INPP5D/SHIP1 (By similarity).
Post-translational modification Glycosylated. Glycosylation plays an inhibitory role in the interaction with IgG3.; The soluble form is produced by a proteolytic cleavage.
Target Relevance information above includes information from UniProt accession: O75015
The UniProt Consortium

Data

SPR with Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein

Biotinylated Human Fc gamma RIIIB (NA2), His Tag captured on CM5 Chip via Anti-his antibody can bind Rituximab with an affinity constant of 3.50 µM as determined in SPR assay (Biacore T200).

HPLC of Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein

The purity of Biotinylated Human Fc gamma RIIIB (NA2) is greater than 95% as determined by SEC-HPLC.

SDS-PAGE gel of Biotinylated Human Fc gamma RIIIB/CD16b (NA2) Protein

Biotinylated Human Fc gamma RIIIB (NA2) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.