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Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein 4724

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Binding assay: Yes (SPR)
  • Amino Acid Range: Ala36-Ile218(R167)
SKU: 4724parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
express system

HEK293

product tag

C-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.

molecular weight

The protein has a predicted MW of 23.2 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein 4724

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma RI (also known as CD64), Fc gamma RII (CD32), and Fc gamma RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10e-8-10e-9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10e-6-10e-7 M) only recognizing IgG aggregates surrounding multivalent antigens.
Protein names
Low affinity immunoglobulin gamma Fc region receptor II-a (IgG Fc receptor II-a) (CDw32) (Fc-gamma RII-a) (Fc-gamma-RIIa) (FcRII-a) (CD antigen CD32)
Gene names
FCGR2A,FCGR2A CD32 FCG2 FCGR2A1 IGFR2
Mass
9606Da
Function
Binds to the Fc region of immunoglobulins gamma. Low affinity receptor. By binding to IgG it initiates cellular responses against pathogens and soluble antigens. Promotes phagocytosis of opsonized antigens.
Subellular location
Cell membrane ; Single-pass type I membrane protein .
Tissues
Found on monocytes, neutrophils and eosinophil platelets.
Structure
Interacts with IGHG1 (PubMed:11711607). Interacts with INPP5D/SHIP1 and INPPL1/SHIP2, regulating its function. Interacts with APCS and FGR. Interacts with HCK.
Post-translational modification
Phosphorylated by SRC-type Tyr-kinases such as LYN, BLK, FYN, HCK and SYK.
Target Relevance information above includes information from UniProt accession: P12318
The UniProt Consortium

Data

SPR with Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein
Biotinylated Human Fc gamma RIIA (R167) , His-Avi Tag captured on CM5 Chip via anti-his antibody can bind Rituximab with an affinity constant of 2.17 µM as determined in SPR assay (Biacore T200) (QC Test).
SPR with Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein
Rituximab captured on CM5 Chip via Protein A can bind Biotinylated Human Fc gamma RIIA (R167) , His-Avi Tag with an affinity constant of 1.03 µM as determined in SPR assay (Biacore T200).
HPLC of Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein
The purity of Biotinylated Fc gamma RIIA (R167) is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human Fc gamma RIIA/CD32a (R167) Protein
Biotinylated Human Fc gamma RIIA (R167) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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Protocols

relevant to this product

Documents

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