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Biotinylated Human ENPP-3 Protein 3332

$525.00$1,750.00

Summary

  • Expression: HEK293
  • Pure: Yes (HPLC)
  • Amino Acid Range: Leu48-Ile875
SKU: 3332parent Categories: , Tag:
Weight1 lbs
Dimensions9 × 5 × 2 in
express system

HEK293

product tag

N-His-Avi

purity

> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC

background

Ectonucleotide pyrophosphatase-phosphodiesterase 3 (ENPP3), a protein detected in the human uterus, has been found to play an important role in the development and invasion of tumours. It was recently discovered that ENPP3 was upregulated during the window of implantation in the human endometrium but its functional relevance remains elusive.

molecular weight

The protein has a predicted MW of 99.97 kDa. Due to glycosylation, the protein migrates to 110-130 kDa based on Tris-Bis PAGE result.

available size

100 µg, 500 µg

endotoxin

Less than 1EU per μg by the LAL method.

Biotinylated Human ENPP-3 Protein 3332

protein
Size and concentration
100, 500µg and lyophilized
Form
Lyophilized
Storage Instructions
Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Storage buffer
Shipped at ambient temperature.
Purity
> 95% as determined by Tris-Bis PAGE
target relevance
Ectonucleotide pyrophosphatase-phosphodiesterase 3 (ENPP3), a protein detected in the human uterus, has been found to play an important role in the development and invasion of tumours. It was recently discovered that ENPP3 was upregulated during the window of implantation in the human endometrium but its functional relevance remains elusive.
Protein names
Ectonucleotide pyrophosphatase/phosphodiesterase family member 3 (E-NPP 3) (NPP3) (Phosphodiesterase I beta) (PD-Ibeta) (Phosphodiesterase I/nucleotide pyrophosphatase 3) (CD antigen CD203c) [Includes: Alkaline phosphodiesterase I (EC 3.1.4.1); Nucleotide pyrophosphatase (NPPase) (EC 3.6.1.9) (Nucleotide diphosphatase)]
Gene names
ENPP3,ENPP3 PDNP3
Protein family
Nucleotide pyrophosphatase/phosphodiesterase family
Mass
9606Da
Function
Hydrolase that metabolizes extracellular nucleotides, including ATP, GTP, UTP and CTP (PubMed:29717535). Limits mast cell and basophil responses during inflammation and during the chronic phases of allergic responses by eliminating the extracellular ATP that functions as signaling molecule and activates basophils and mast cells and induces the release of inflammatory cytokines. Metabolizes extracellular ATP in the lumen of the small intestine, and thereby prevents ATP-induced apoptosis of intestinal plasmacytoid dendritic cells (By similarity). Has also alkaline phosphodiesterase activity (PubMed:11342463).
Catalytic activity
BINDING 167; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /ligand_note="catalytic"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01, ECO:0007744|PDB:6C02"; BINDING 204; /ligand="substrate"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C02"; BINDING 205; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /ligand_note="catalytic"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01"; BINDING 226; /ligand="substrate"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C02"; BINDING 275; /ligand="substrate"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C02"; BINDING 289; /ligand="substrate"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C02"; BINDING 325; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /ligand_note="catalytic"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01, ECO:0007744|PDB:6C02"; BINDING 329; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /ligand_note="catalytic"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01, ECO:0007744|PDB:6C02"; BINDING 372; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /ligand_note="catalytic"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01, ECO:0007744|PDB:6C02"; BINDING 373; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="1"; /ligand_note="catalytic"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01, ECO:0007744|PDB:6C02"; BINDING 483; /ligand="Zn(2+)"; /ligand_id="ChEBI:CHEBI:29105"; /ligand_label="2"; /ligand_note="catalytic"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01, ECO:0007744|PDB:6C02"; BINDING 752; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01, ECO:0007744|PDB:6C02"; BINDING 754; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01, ECO:0007744|PDB:6C02"; BINDING 756; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01, ECO:0007744|PDB:6C02"; BINDING 758; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01, ECO:0007744|PDB:6C02"; BINDING 760; /ligand="Ca(2+)"; /ligand_id="ChEBI:CHEBI:29108"; /evidence="ECO:0000269|PubMed:29717535, ECO:0007744|PDB:6C01, ECO:0007744|PDB:6C02"
Subellular location
Cell membrane ; Single-pass type II membrane protein. Apical cell membrane ; Single-pass type II membrane protein. Secreted. Note=Detected at the cell surface of basophils (PubMed:11342463). Detected at the apical plasma membrane of bile duct cells (PubMed:15072822). Located to the apical surface in intestinal and kidney epithelial cells. Secreted in serum, and in lumen of epithelial cells.
Tissues
Detected on bile ducts in liver, and in blood serum (at protein level) (PubMed:15072822). Detected in prostate and uterus (PubMed:9344668). Detected on basophils, but not neutrophils (PubMed:11342463).
Structure
Monomer and homodimer.
Post-translational modification
N-glycosylated. N-glycosylation is necessary for normal transport to the cell membrane, but is not the apical targeting signal.
Target Relevance information above includes information from UniProt accession: O14638
The UniProt Consortium

Data

HPLC of Biotinylated Human ENPP-3 Protein
The purity of Biotinylated Human ENPP-3 is greater than 95% as determined by SEC-HPLC.
SDS-PAGE gel of Biotinylated Human ENPP-3 Protein
Biotinylated Human ENPP-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

Publications

Published literature highly relevant to the biological target of this product and referencing this antibody or clone are retrieved from PubMed database provided by The United States National Library of Medicine at the National Institutes of Health.




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